Cyclopropanation Reactions by a Class I Unspecific Peroxygenase

Gideon James Grogan; Jason Li; Katy Cornish; Balazs Pogranyi; Benjamin Melling; Jared Cartwright; William Paul Unsworth

doi:10.1039/D5OB00426H

Cyclopropanation Reactions by a Class I Unspecific Peroxygenase

Gideon James Grogan, Jason Li, Katy Cornish, Balazs Pogranyi, Benjamin Melling, Jared Cartwright, William Paul Unsworth

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Non-natural biotransformations, such as alkene cyclopropanation through carbene insertion, have been demonstrated for the hemoproteins cytochrome P450 and myoglobin, but have not been investigated for Unspecific Peroxygenases (UPOs). Here we demonstrate that the diastereo- and enantioselective cyclopropanation of styrenes with ethyldiazoacetate can be performed by the Class I enzyme artUPO.

Original language	English
Number of pages	7
Journal	Organic and Biomolecular Chemistry
Early online date	17 Apr 2025
DOIs	https://doi.org/10.1039/D5OB00426H
Publication status	E-pub ahead of print - 17 Apr 2025

Bibliographical note

This is an author-produced version of the published paper. Uploaded in accordance with the University’s Research Publications and Open Access policy.

Access to Document

10.1039/D5OB00426H

Accepted_Manuscript
This is an author-produced version of the published paper. Uploaded in accordance with the University’s Research Publications and Open Access policy.
Accepted author manuscript, 1.09 MBLicence: CC BY

Cite this

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abstract = "Non-natural biotransformations, such as alkene cyclopropanation through carbene insertion, have been demonstrated for the hemoproteins cytochrome P450 and myoglobin, but have not been investigated for Unspecific Peroxygenases (UPOs). Here we demonstrate that the diastereo- and enantioselective cyclopropanation of styrenes with ethyldiazoacetate can be performed by the Class I enzyme artUPO.",

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AU - Li, Jason

AU - Cornish, Katy

AU - Pogranyi, Balazs

AU - Melling, Benjamin

AU - Cartwright, Jared

AU - Unsworth, William Paul

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AB - Non-natural biotransformations, such as alkene cyclopropanation through carbene insertion, have been demonstrated for the hemoproteins cytochrome P450 and myoglobin, but have not been investigated for Unspecific Peroxygenases (UPOs). Here we demonstrate that the diastereo- and enantioselective cyclopropanation of styrenes with ethyldiazoacetate can be performed by the Class I enzyme artUPO.

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