Cytomorphological changes of oral mucosal cells among smokeless tobacco users in low and middle-income country settings: new findings from Pakistan

Sabreen Hassan; Ayesha Sifat; Mohammad Munib; Saleha Saeed; Waqar U. Nisa; Sofia Haider Durrani; Abid Rahim; Naeem Ullah; Saima Afaq; Farhad Ali Khattak; Zia Ul Haq

doi:10.1186/s12903-024-05220-7

Cytomorphological changes of oral mucosal cells among smokeless tobacco users in low and middle-income country settings: new findings from Pakistan

Sabreen Hassan, Ayesha Sifat, Mohammad Munib, Saleha Saeed, Waqar U. Nisa, Sofia Haider Durrani, Abid Rahim, Naeem Ullah, Saima Afaq^*, Farhad Ali Khattak^*, Zia Ul Haq

^*Corresponding author for this work

Health Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Chronic tobacco use, in any form, induces significant cellular alterations in the oral mucosa. This study investigates four distinct cytomorphological changes in oral mucosal cells among smokeless tobacco users, examining their association across different genders and age groups. Materials and methods: This cross-sectional study involved collecting mucosal samples from smokeless tobacco (naswar/snuff) users through consecutive sampling. The prepared smears were examined for dysplastic changes. Data analysis was performed using SPSS, with Chi-square tests and logistic regression employed to evaluate proportions and associations. Results: Among 100 Naswar/snuff users, the labial sulcus mucosa was the most common site affected (47%). The most frequent cytomorphological change was micronuclei (46%), followed by nuclear budding (25%), perinuclear halo (18%), and binucleated cells (14%). In the 51–60 age group, micronuclei (78.9%), nuclear budding (55.3%), binucleation (31.6%), and perinuclear halo (36.8%) were more prevalent (P < 0.005). Gender analysis revealed that micronuclei were more common in females (54.2%) compared to males (43.4%), while nuclear budding was more prevalent in males (27.6%) than females (P < 0.28). Logistic regression indicated that individuals aged 51–60 were more likely to exhibit micronuclei (OR = 1.15, 95% CI: 0.22 to 5.83, P = 0.863) and nuclear budding (OR = 15.34, 95% CI: 9.23 to 30.75, P < 0.05). Conclusion: The dysplastic changes observed included micronuclei, nuclear budding, binucleated cells, and perinuclear halo, with micronuclei being the most prevalent. These findings could facilitate the early diagnosis of oral lesions and their timely management in habitual smokeless tobacco users.

Original language	English
Article number	1541
Number of pages	8
Journal	BMC Oral Health
Volume	24
Issue number	1
DOIs	https://doi.org/10.1186/s12903-024-05220-7 https://doi.org/10.1186/s12903-024-05220-7
Publication status	Published - 23 Dec 2024

Bibliographical note

Publisher Copyright:
© The Author(s) 2024.

Keywords

Cellular alterations
Micronuclei
Naswar/snuff
Oral cancer
Oral lesions
Smokeless tobacco

Access to Document

Cytomorphological changes of oral mucosal cells among smokeless tobacco users in low and middle-income country settings: new fndings from Pakistan
© The Author(s) 2024
Final published version, 829 KBLicence: CC BY

Cite this

Hassan, S., Sifat, A., Munib, M., Saeed, S., Nisa, W. U., Durrani, S. H., Rahim, A., Ullah, N., Afaq, S., Ali Khattak, F., & Haq, Z. U. (2024). Cytomorphological changes of oral mucosal cells among smokeless tobacco users in low and middle-income country settings: new findings from Pakistan. BMC Oral Health, 24(1), Article 1541. https://doi.org/10.1186/s12903-024-05220-7, https://doi.org/10.1186/s12903-024-05220-7

@article{07e34b67cd2b4a098f276b10125fbc84,

title = "Cytomorphological changes of oral mucosal cells among smokeless tobacco users in low and middle-income country settings: new findings from Pakistan",

abstract = "Background: Chronic tobacco use, in any form, induces significant cellular alterations in the oral mucosa. This study investigates four distinct cytomorphological changes in oral mucosal cells among smokeless tobacco users, examining their association across different genders and age groups. Materials and methods: This cross-sectional study involved collecting mucosal samples from smokeless tobacco (naswar/snuff) users through consecutive sampling. The prepared smears were examined for dysplastic changes. Data analysis was performed using SPSS, with Chi-square tests and logistic regression employed to evaluate proportions and associations. Results: Among 100 Naswar/snuff users, the labial sulcus mucosa was the most common site affected (47%). The most frequent cytomorphological change was micronuclei (46%), followed by nuclear budding (25%), perinuclear halo (18%), and binucleated cells (14%). In the 51–60 age group, micronuclei (78.9%), nuclear budding (55.3%), binucleation (31.6%), and perinuclear halo (36.8%) were more prevalent (P < 0.005). Gender analysis revealed that micronuclei were more common in females (54.2%) compared to males (43.4%), while nuclear budding was more prevalent in males (27.6%) than females (P < 0.28). Logistic regression indicated that individuals aged 51–60 were more likely to exhibit micronuclei (OR = 1.15, 95% CI: 0.22 to 5.83, P = 0.863) and nuclear budding (OR = 15.34, 95% CI: 9.23 to 30.75, P < 0.05). Conclusion: The dysplastic changes observed included micronuclei, nuclear budding, binucleated cells, and perinuclear halo, with micronuclei being the most prevalent. These findings could facilitate the early diagnosis of oral lesions and their timely management in habitual smokeless tobacco users.",

keywords = "Cellular alterations, Micronuclei, Naswar/snuff, Oral cancer, Oral lesions, Smokeless tobacco",

author = "Sabreen Hassan and Ayesha Sifat and Mohammad Munib and Saleha Saeed and Nisa, {Waqar U.} and Durrani, {Sofia Haider} and Abid Rahim and Naeem Ullah and Saima Afaq and {Ali Khattak}, Farhad and Haq, {Zia Ul}",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2024.",

year = "2024",

month = dec,

day = "23",

doi = "10.1186/s12903-024-05220-7",

language = "English",

volume = "24",

journal = "BMC Oral Health",

issn = "1472-6831",

publisher = "Springer",

number = "1",

}

Hassan, S, Sifat, A, Munib, M, Saeed, S, Nisa, WU, Durrani, SH, Rahim, A, Ullah, N, Afaq, S, Ali Khattak, F & Haq, ZU 2024, 'Cytomorphological changes of oral mucosal cells among smokeless tobacco users in low and middle-income country settings: new findings from Pakistan', BMC Oral Health, vol. 24, no. 1, 1541. https://doi.org/10.1186/s12903-024-05220-7, https://doi.org/10.1186/s12903-024-05220-7

TY - JOUR

T1 - Cytomorphological changes of oral mucosal cells among smokeless tobacco users in low and middle-income country settings

T2 - new findings from Pakistan

AU - Hassan, Sabreen

AU - Sifat, Ayesha

AU - Munib, Mohammad

AU - Saeed, Saleha

AU - Nisa, Waqar U.

AU - Durrani, Sofia Haider

AU - Rahim, Abid

AU - Ullah, Naeem

AU - Afaq, Saima

AU - Ali Khattak, Farhad

AU - Haq, Zia Ul

PY - 2024/12/23

Y1 - 2024/12/23

N2 - Background: Chronic tobacco use, in any form, induces significant cellular alterations in the oral mucosa. This study investigates four distinct cytomorphological changes in oral mucosal cells among smokeless tobacco users, examining their association across different genders and age groups. Materials and methods: This cross-sectional study involved collecting mucosal samples from smokeless tobacco (naswar/snuff) users through consecutive sampling. The prepared smears were examined for dysplastic changes. Data analysis was performed using SPSS, with Chi-square tests and logistic regression employed to evaluate proportions and associations. Results: Among 100 Naswar/snuff users, the labial sulcus mucosa was the most common site affected (47%). The most frequent cytomorphological change was micronuclei (46%), followed by nuclear budding (25%), perinuclear halo (18%), and binucleated cells (14%). In the 51–60 age group, micronuclei (78.9%), nuclear budding (55.3%), binucleation (31.6%), and perinuclear halo (36.8%) were more prevalent (P < 0.005). Gender analysis revealed that micronuclei were more common in females (54.2%) compared to males (43.4%), while nuclear budding was more prevalent in males (27.6%) than females (P < 0.28). Logistic regression indicated that individuals aged 51–60 were more likely to exhibit micronuclei (OR = 1.15, 95% CI: 0.22 to 5.83, P = 0.863) and nuclear budding (OR = 15.34, 95% CI: 9.23 to 30.75, P < 0.05). Conclusion: The dysplastic changes observed included micronuclei, nuclear budding, binucleated cells, and perinuclear halo, with micronuclei being the most prevalent. These findings could facilitate the early diagnosis of oral lesions and their timely management in habitual smokeless tobacco users.

AB - Background: Chronic tobacco use, in any form, induces significant cellular alterations in the oral mucosa. This study investigates four distinct cytomorphological changes in oral mucosal cells among smokeless tobacco users, examining their association across different genders and age groups. Materials and methods: This cross-sectional study involved collecting mucosal samples from smokeless tobacco (naswar/snuff) users through consecutive sampling. The prepared smears were examined for dysplastic changes. Data analysis was performed using SPSS, with Chi-square tests and logistic regression employed to evaluate proportions and associations. Results: Among 100 Naswar/snuff users, the labial sulcus mucosa was the most common site affected (47%). The most frequent cytomorphological change was micronuclei (46%), followed by nuclear budding (25%), perinuclear halo (18%), and binucleated cells (14%). In the 51–60 age group, micronuclei (78.9%), nuclear budding (55.3%), binucleation (31.6%), and perinuclear halo (36.8%) were more prevalent (P < 0.005). Gender analysis revealed that micronuclei were more common in females (54.2%) compared to males (43.4%), while nuclear budding was more prevalent in males (27.6%) than females (P < 0.28). Logistic regression indicated that individuals aged 51–60 were more likely to exhibit micronuclei (OR = 1.15, 95% CI: 0.22 to 5.83, P = 0.863) and nuclear budding (OR = 15.34, 95% CI: 9.23 to 30.75, P < 0.05). Conclusion: The dysplastic changes observed included micronuclei, nuclear budding, binucleated cells, and perinuclear halo, with micronuclei being the most prevalent. These findings could facilitate the early diagnosis of oral lesions and their timely management in habitual smokeless tobacco users.

KW - Cellular alterations

KW - Micronuclei

KW - Naswar/snuff

KW - Oral cancer

KW - Oral lesions

KW - Smokeless tobacco

UR - http://www.scopus.com/inward/record.url?scp=85212765853&partnerID=8YFLogxK

U2 - 10.1186/s12903-024-05220-7

DO - 10.1186/s12903-024-05220-7

M3 - Article

C2 - 39710646

AN - SCOPUS:85212765853

SN - 1472-6831

VL - 24

JO - BMC Oral Health

JF - BMC Oral Health

IS - 1

M1 - 1541

ER -

Cytomorphological changes of oral mucosal cells among smokeless tobacco users in low and middle-income country settings: new findings from Pakistan

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Cite this