By the same authors

From the same journal

From the same journal

Deep Sequencing of RNA from Ancient Maize Kernels

Research output: Contribution to journalArticle

Published copy (DOI)

Author(s)

  • Sarah L. Fordyce
  • Maria C. Ávila-Arcos
  • Morten Rasmussen
  • Enrico Cappellini
  • J. Alberto Romero-Navarro
  • Nathan Wales
  • David E. Alquezar-Planas
  • Steven Penfield
  • Terence A. Brown
  • Jean Philippe Vielle-Calzada
  • Rafael Montiel
  • Tina Jørgensen
  • Nancy Odegaard
  • Michael Jacobs
  • Bernardo Arriaza
  • Thomas F. G. Higham
  • Christopher Bronk Ramsey
  • Eske Willerslev
  • M. Thomas P. Gilbert

Department/unit(s)

Publication details

JournalPLoS ONE
DatePublished - 17 Jan 2013
Issue number1
Volume8
Original languageEnglish

Abstract

The characterization of biomolecules from ancient samples can shed otherwise unobtainable insights into the past. Despite the fundamental role of transcriptomal change in evolution, the potential of ancient RNA remains unexploited - perhaps due to dogma associated with the fragility of RNA. We hypothesize that seeds offer a plausible refuge for long-term RNA survival, due to the fundamental role of RNA during seed germination. Using RNA-Seq on cDNA synthesized from nucleic acid extracts, we validate this hypothesis through demonstration of partial transcriptomal recovery from two sources of ancient maize kernels. The results suggest that ancient seed transcriptomics may offer a powerful new tool with which to study plant domestication.

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations