Delivery of nitric oxide for analysis of the function of cytochrome c'

Lindsay J. Cole; Wilhelmina M. Huston; James W. B. Moir

doi:10.1016/S0076-6879(08)36002-9

Delivery of nitric oxide for analysis of the function of cytochrome c'

Lindsay J. Cole, Wilhelmina M. Huston, James W. B. Moir

Biology

Research output: Chapter in Book/Report/Conference proceeding › Other chapter contribution

Abstract

On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c'), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady-state UV/Vis spectrophotometry and pre-steady-state stopped-flow spectrophotometry analysis of cytochrome c'.

Original language	English
Title of host publication	GLOBINS AND OTHER NITRIC OXIDE-REACTIVE PROTEINS, PT A
Place of Publication	SAN DIEGO
Publisher	Elsevier
Pages	21-33
Number of pages	13
DOIs	https://doi.org/10.1016/S0076-6879(08)36002-9
Publication status	Published - 2008

Keywords

SOLUBLE GUANYLATE-CYCLASE
RHODOBACTER-CAPSULATUS
HEME
KINETICS
BINDING

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10.1016/S0076-6879(08)36002-9

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abstract = "On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c'), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady-state UV/Vis spectrophotometry and pre-steady-state stopped-flow spectrophotometry analysis of cytochrome c'.",

keywords = "SOLUBLE GUANYLATE-CYCLASE, RHODOBACTER-CAPSULATUS, HEME, KINETICS, BINDING",

author = "Cole, {Lindsay J.} and Huston, {Wilhelmina M.} and Moir, {James W. B.}",

year = "2008",

doi = "10.1016/S0076-6879(08)36002-9",

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T1 - Delivery of nitric oxide for analysis of the function of cytochrome c'

AU - Cole, Lindsay J.

AU - Huston, Wilhelmina M.

AU - Moir, James W. B.

PY - 2008

Y1 - 2008

N2 - On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c'), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady-state UV/Vis spectrophotometry and pre-steady-state stopped-flow spectrophotometry analysis of cytochrome c'.

AB - On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c'), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady-state UV/Vis spectrophotometry and pre-steady-state stopped-flow spectrophotometry analysis of cytochrome c'.

KW - SOLUBLE GUANYLATE-CYCLASE

KW - RHODOBACTER-CAPSULATUS

KW - HEME

KW - KINETICS

KW - BINDING

UR - http://www.scopus.com/inward/record.url?scp=42949096499&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(08)36002-9

DO - 10.1016/S0076-6879(08)36002-9

M3 - Other chapter contribution

SP - 21

EP - 33

BT - GLOBINS AND OTHER NITRIC OXIDE-REACTIVE PROTEINS, PT A

PB - Elsevier

CY - SAN DIEGO

ER -

Delivery of nitric oxide for analysis of the function of cytochrome c'

Abstract

Keywords

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