Delivery of nitric oxide for analysis of the function of cytochrome c'

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Publication details

Title of host publicationGLOBINS AND OTHER NITRIC OXIDE-REACTIVE PROTEINS, PT A
DatePublished - 2008
Pages21-33
Number of pages13
PublisherELSEVIER ACADEMIC PRESS INC
Place of PublicationSAN DIEGO
Original languageEnglish

Abstract

On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c'), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady-state UV/Vis spectrophotometry and pre-steady-state stopped-flow spectrophotometry analysis of cytochrome c'.

    Research areas

  • SOLUBLE GUANYLATE-CYCLASE, RHODOBACTER-CAPSULATUS, HEME, KINETICS, BINDING

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