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DELLA-interacting SWI3C core subunit of switch/sucrose nonfermenting chromatin remodeling complex modulates gibberellin responses and hormonal cross talk in Arabidopsis

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Published copy (DOI)

Author(s)

  • Elzbieta A Sarnowska
  • Anna T Rolicka
  • Ernest Bucior
  • Pawel Cwiek
  • Takayuki Tohge
  • Alisdair R Fernie
  • Yusuke Jikumaru
  • Yuji Kamiya
  • Rainer Franzen
  • Elmon Schmelzer
  • Aimone Porri
  • Sebastian Sacharowski
  • Dominika M Gratkowska
  • Dorota L Zugaj
  • Aleksandra Taff
  • Anna Zalewska
  • Rafal Archacki
  • George Coupland
  • Csaba Koncz
  • Andrzej Jerzmanowski
  • Tomasz J Sarnowski

Department/unit(s)

Publication details

JournalPlant Physiology
DateE-pub ahead of print - 1 Jul 2013
DatePublished (current) - Sep 2013
Issue number1
Volume163
Number of pages13
Pages (from-to)305-317
Early online date1/07/13
Original languageEnglish

Abstract

Switch (SWI)/Sucrose Nonfermenting (SNF)-type chromatin-remodeling complexes (CRCs) are involved in regulation of transcription, DNA replication and repair, and cell cycle. Mutations of conserved subunits of plant CRCs severely impair growth and development; however, the underlying causes of these phenotypes are largely unknown. Here, we show that inactivation of SWI3C, the core component of Arabidopsis (Arabidopsis thaliana) SWI/SNF CRCs, interferes with normal functioning of several plant hormone pathways and alters transcriptional regulation of key genes of gibberellin (GA) biosynthesis. The resulting reduction of GA4 causes severe inhibition of hypocotyl and root elongation, which can be rescued by exogenous GA treatment. In addition, the swi3c mutation inhibits DELLA-dependent transcriptional activation of GIBBERELLIN-INSENSITIVE DWARF1 (GID1) GA receptor genes. Down-regulation of GID1a in parallel with the DELLA repressor gene REPRESSOR OF GA1-3 1 in swi3c indicates that lack of SWI3C also leads to defects in GA signaling. Together with the recent demonstration of function of SWI/SNF ATPase BRAHMA in the GA pathway, these results reveal a critical role of SWI/SNF CRC in the regulation of GA biosynthesis and signaling. Moreover, we demonstrate that SWI3C is capable of in vitro binding to, and shows in vivo bimolecular fluorescence complementation interaction in cell nuclei with, the DELLA proteins RGA-LIKE2 and RGA-LIKE3, which affect transcriptional activation of GID1 and GA3ox (GIBBERELLIN 3-OXIDASE) genes controlling GA perception and biosynthesis, respectively. Furthermore, we show that SWI3C also interacts with the O-GlcNAc (O-linked N-acetylglucosamine) transferase SPINDLY required for proper functioning of DELLAs and acts hypostatically to (SPINDLY) in the GA response pathway. These findings suggest that DELLA-mediated effects in GA signaling as well as their role as a hub in hormonal cross talk may be, at least in part, dependent on their direct physical interaction with complexes responsible for modulation of chromatin structure.

Bibliographical note

©2013 American Society of Plant Biologists. All rights reserved.

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