Projects per year
Abstract
Binding of drugs to blood serum proteins can influence both therapeutic efficacy and toxicity. The ability to measure the concentrations of protein-bound drug molecules quickly and with limited sample preparation could therefore have considerable benefits in biomedical and pharmaceutical applications. Vibrational spectroscopies provide data quickly but are hampered by complex, overlapping protein amide I band profiles and water absorption. Here, we show that two-dimensional infrared (2D-IR) spectroscopy can achieve rapid detection and quantification of paracetamol binding to serum albumin in blood serum at physiologically-relevant levels with no additional sample processing. By measuring changes to the amide I band of serum albumin caused by structural and dynamic impacts of paracetamol binding we show that drug concentrations as low as 7 µM can be detected and that the availability of albumin for paracetamol binding is less than 20% in serum samples, allowing identification of paracetamol levels consistent with a patient overdose.
Original language | English |
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Pages (from-to) | 3464-3469 |
Number of pages | 6 |
Journal | Analyst |
Volume | 147 |
DOIs | |
Publication status | Published - 14 Jul 2022 |
Bibliographical note
© The Royal Society of Chemistry 2022Projects
- 1 Finished
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EPSRC Responsive -2D-IR spectroscopy for serum diagnostics
1/10/20 → 31/03/24
Project: Research project (funded) › Research