Determination of Muscle Fiber Type in Rodents

Research output: Chapter in Book/Report/Conference proceedingChapter

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Determination of Muscle Fiber Type in Rodents. / Kalmar, Bernadett; Blanco, Gonzalo; Greensmith, Linda.

Current Protocols in Mouse Biology. 2012. p. 231.

Research output: Chapter in Book/Report/Conference proceedingChapter

Harvard

Kalmar, B, Blanco, G & Greensmith, L 2012, Determination of Muscle Fiber Type in Rodents. in Current Protocols in Mouse Biology. pp. 231. https://doi.org/10.1002/9780470942390.mo110229

APA

Kalmar, B., Blanco, G., & Greensmith, L. (2012). Determination of Muscle Fiber Type in Rodents. In Current Protocols in Mouse Biology (pp. 231) https://doi.org/10.1002/9780470942390.mo110229

Vancouver

Kalmar B, Blanco G, Greensmith L. Determination of Muscle Fiber Type in Rodents. In Current Protocols in Mouse Biology. 2012. p. 231 https://doi.org/10.1002/9780470942390.mo110229

Author

Kalmar, Bernadett ; Blanco, Gonzalo ; Greensmith, Linda. / Determination of Muscle Fiber Type in Rodents. Current Protocols in Mouse Biology. 2012. pp. 231

Bibtex - Download

@inbook{c4c09ad6cd9f4b4790a7cf7f684d7155,
title = "Determination of Muscle Fiber Type in Rodents",
abstract = "Skeletal muscles consist of muscle fibers that can differ in both composition and functional characteristics. These three types of muscle fibers, broadly categorized as slow, fast IIa, and fast IIb muscle fibers, express characteristic myosin heavy chain proteins and have different metabolic and enzymatic activities, which can be used as surrogate markers to identify the different fiber types. Pathological changes affecting the muscle, such as denervation, muscle disuse, and atrophy not only manifest on a functional level, but also as marked changes in the composition of muscle fiber type of individual muscles. In this unit we describe three methods for histological identification of slow/type I, fast fatigue resistant/type IIa, and fast fatigable/type IIb fibers by staining for either myosin ATPases or oxidative enzyme capacity (succinate dehydrogenase, SDH)—or, alternatively, immunostaining for specific myosin heavy chain isoforms in muscles of mouse hindlimbs. Curr. Protoc. Mouse Biol. 2:231-243 {\textcopyright} 2012 by John Wiley & Sons, Inc.",
author = "Bernadett Kalmar and Gonzalo Blanco and Linda Greensmith",
year = "2012",
month = sep,
day = "1",
doi = "10.1002/9780470942390.mo110229",
language = "English",
pages = "231",
booktitle = "Current Protocols in Mouse Biology",

}

RIS (suitable for import to EndNote) - Download

TY - CHAP

T1 - Determination of Muscle Fiber Type in Rodents

AU - Kalmar, Bernadett

AU - Blanco, Gonzalo

AU - Greensmith, Linda

PY - 2012/9/1

Y1 - 2012/9/1

N2 - Skeletal muscles consist of muscle fibers that can differ in both composition and functional characteristics. These three types of muscle fibers, broadly categorized as slow, fast IIa, and fast IIb muscle fibers, express characteristic myosin heavy chain proteins and have different metabolic and enzymatic activities, which can be used as surrogate markers to identify the different fiber types. Pathological changes affecting the muscle, such as denervation, muscle disuse, and atrophy not only manifest on a functional level, but also as marked changes in the composition of muscle fiber type of individual muscles. In this unit we describe three methods for histological identification of slow/type I, fast fatigue resistant/type IIa, and fast fatigable/type IIb fibers by staining for either myosin ATPases or oxidative enzyme capacity (succinate dehydrogenase, SDH)—or, alternatively, immunostaining for specific myosin heavy chain isoforms in muscles of mouse hindlimbs. Curr. Protoc. Mouse Biol. 2:231-243 © 2012 by John Wiley & Sons, Inc.

AB - Skeletal muscles consist of muscle fibers that can differ in both composition and functional characteristics. These three types of muscle fibers, broadly categorized as slow, fast IIa, and fast IIb muscle fibers, express characteristic myosin heavy chain proteins and have different metabolic and enzymatic activities, which can be used as surrogate markers to identify the different fiber types. Pathological changes affecting the muscle, such as denervation, muscle disuse, and atrophy not only manifest on a functional level, but also as marked changes in the composition of muscle fiber type of individual muscles. In this unit we describe three methods for histological identification of slow/type I, fast fatigue resistant/type IIa, and fast fatigable/type IIb fibers by staining for either myosin ATPases or oxidative enzyme capacity (succinate dehydrogenase, SDH)—or, alternatively, immunostaining for specific myosin heavy chain isoforms in muscles of mouse hindlimbs. Curr. Protoc. Mouse Biol. 2:231-243 © 2012 by John Wiley & Sons, Inc.

U2 - 10.1002/9780470942390.mo110229

DO - 10.1002/9780470942390.mo110229

M3 - Chapter

SP - 231

BT - Current Protocols in Mouse Biology

ER -