Development and characterisation of a full-thickness acellular porcine bladder matrix for tissue engineering

Fiona Bolland; Sotiris Korossis; Stacy-Paul Wilshaw; Eileen Ingham; John Fisher; John N Kearney; Jennifer Southgate

doi:10.1016/j.biomaterials.2006.10.005

Development and characterisation of a full-thickness acellular porcine bladder matrix for tissue engineering

Fiona Bolland, Sotiris Korossis, Stacy-Paul Wilshaw, Eileen Ingham, John Fisher, John N Kearney, Jennifer Southgate

Jack Birch Unit

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this study was to produce a natural, acellular matrix from porcine bladder tissue for use as a scaffold in developing a tissue-engineered bladder replacement. Full-thickness, intact porcine bladders were decellularised by distention and immersion in hypotonic buffer containing 0.1% (w/v) SDS and nuclease enzymes. Histological analysis of the resultant matrices showed they were completely acellular; that the major structural proteins had been retained and that there were some residual poorly soluble intracellular proteins. The amount of DNA per mg dry weight of fresh porcine bladder was 2.8 (+/- 0.1) mu g/mg compared to 0.1 (+/- 0.1) mu g/mg in decellularised bladder and biochemical analysis showed proportional differences in the hydroxyproline and glycosaminoglycan content of the tissue before and after decellularisation. Uniaxial tensile testing indicated that decellularisation did not significantly compromise the ultimate tensile strength of the tissue. There was, however, an increase in the collagen and elastin phase slopes indicating decreased extensibility. Cytotoxicity assays using porcine smooth muscle cell cultures excluded the presence of soluble toxins in the biomaterial. In summary, a full-thickness natural acellular matrix retaining the major structural components and strength of the urinary bladder has been successfully developed. The matrix is biocompatible with bladder-derived cells and has potential for use in urological surgery and tissue-engineering applications. (c) 2006 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	1061-1070
Number of pages	10
Journal	Biomaterials
Volume	28
Issue number	6
DOIs	https://doi.org/10.1016/j.biomaterials.2006.10.005
Publication status	Published - Feb 2007

Keywords

bladder tissue engineering
mechanical properties
scaffold
smooth muscle cells
SMALL-INTESTINAL SUBMUCOSA
HUMAN UROTHELIAL CELLS
URINARY-BLADDER
SULFATED GLYCOSAMINOGLYCANS
EXTRACELLULAR-MATRIX
IN-VITRO
HYDROXYPROLINE
RECONSTRUCTION
AUGMENTATION
MODEL

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10.1016/j.biomaterials.2006.10.005

Cite this

@article{6775616d35e84f559ae66b75efe29d2c,

title = "Development and characterisation of a full-thickness acellular porcine bladder matrix for tissue engineering",

abstract = "The aim of this study was to produce a natural, acellular matrix from porcine bladder tissue for use as a scaffold in developing a tissue-engineered bladder replacement. Full-thickness, intact porcine bladders were decellularised by distention and immersion in hypotonic buffer containing 0.1% (w/v) SDS and nuclease enzymes. Histological analysis of the resultant matrices showed they were completely acellular; that the major structural proteins had been retained and that there were some residual poorly soluble intracellular proteins. The amount of DNA per mg dry weight of fresh porcine bladder was 2.8 (+/- 0.1) mu g/mg compared to 0.1 (+/- 0.1) mu g/mg in decellularised bladder and biochemical analysis showed proportional differences in the hydroxyproline and glycosaminoglycan content of the tissue before and after decellularisation. Uniaxial tensile testing indicated that decellularisation did not significantly compromise the ultimate tensile strength of the tissue. There was, however, an increase in the collagen and elastin phase slopes indicating decreased extensibility. Cytotoxicity assays using porcine smooth muscle cell cultures excluded the presence of soluble toxins in the biomaterial. In summary, a full-thickness natural acellular matrix retaining the major structural components and strength of the urinary bladder has been successfully developed. The matrix is biocompatible with bladder-derived cells and has potential for use in urological surgery and tissue-engineering applications. (c) 2006 Elsevier Ltd. All rights reserved.",

keywords = "bladder tissue engineering, mechanical properties, scaffold, smooth muscle cells, SMALL-INTESTINAL SUBMUCOSA, HUMAN UROTHELIAL CELLS, URINARY-BLADDER, SULFATED GLYCOSAMINOGLYCANS, EXTRACELLULAR-MATRIX, IN-VITRO, HYDROXYPROLINE, RECONSTRUCTION, AUGMENTATION, MODEL",

author = "Fiona Bolland and Sotiris Korossis and Stacy-Paul Wilshaw and Eileen Ingham and John Fisher and Kearney, {John N} and Jennifer Southgate",

year = "2007",

month = feb,

doi = "10.1016/j.biomaterials.2006.10.005",

language = "English",

volume = "28",

pages = "1061--1070",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier",

number = "6",

}

TY - JOUR

T1 - Development and characterisation of a full-thickness acellular porcine bladder matrix for tissue engineering

AU - Bolland, Fiona

AU - Korossis, Sotiris

AU - Wilshaw, Stacy-Paul

AU - Ingham, Eileen

AU - Fisher, John

AU - Kearney, John N

AU - Southgate, Jennifer

PY - 2007/2

Y1 - 2007/2

N2 - The aim of this study was to produce a natural, acellular matrix from porcine bladder tissue for use as a scaffold in developing a tissue-engineered bladder replacement. Full-thickness, intact porcine bladders were decellularised by distention and immersion in hypotonic buffer containing 0.1% (w/v) SDS and nuclease enzymes. Histological analysis of the resultant matrices showed they were completely acellular; that the major structural proteins had been retained and that there were some residual poorly soluble intracellular proteins. The amount of DNA per mg dry weight of fresh porcine bladder was 2.8 (+/- 0.1) mu g/mg compared to 0.1 (+/- 0.1) mu g/mg in decellularised bladder and biochemical analysis showed proportional differences in the hydroxyproline and glycosaminoglycan content of the tissue before and after decellularisation. Uniaxial tensile testing indicated that decellularisation did not significantly compromise the ultimate tensile strength of the tissue. There was, however, an increase in the collagen and elastin phase slopes indicating decreased extensibility. Cytotoxicity assays using porcine smooth muscle cell cultures excluded the presence of soluble toxins in the biomaterial. In summary, a full-thickness natural acellular matrix retaining the major structural components and strength of the urinary bladder has been successfully developed. The matrix is biocompatible with bladder-derived cells and has potential for use in urological surgery and tissue-engineering applications. (c) 2006 Elsevier Ltd. All rights reserved.

AB - The aim of this study was to produce a natural, acellular matrix from porcine bladder tissue for use as a scaffold in developing a tissue-engineered bladder replacement. Full-thickness, intact porcine bladders were decellularised by distention and immersion in hypotonic buffer containing 0.1% (w/v) SDS and nuclease enzymes. Histological analysis of the resultant matrices showed they were completely acellular; that the major structural proteins had been retained and that there were some residual poorly soluble intracellular proteins. The amount of DNA per mg dry weight of fresh porcine bladder was 2.8 (+/- 0.1) mu g/mg compared to 0.1 (+/- 0.1) mu g/mg in decellularised bladder and biochemical analysis showed proportional differences in the hydroxyproline and glycosaminoglycan content of the tissue before and after decellularisation. Uniaxial tensile testing indicated that decellularisation did not significantly compromise the ultimate tensile strength of the tissue. There was, however, an increase in the collagen and elastin phase slopes indicating decreased extensibility. Cytotoxicity assays using porcine smooth muscle cell cultures excluded the presence of soluble toxins in the biomaterial. In summary, a full-thickness natural acellular matrix retaining the major structural components and strength of the urinary bladder has been successfully developed. The matrix is biocompatible with bladder-derived cells and has potential for use in urological surgery and tissue-engineering applications. (c) 2006 Elsevier Ltd. All rights reserved.

KW - bladder tissue engineering

KW - mechanical properties

KW - scaffold

KW - smooth muscle cells

KW - SMALL-INTESTINAL SUBMUCOSA

KW - HUMAN UROTHELIAL CELLS

KW - URINARY-BLADDER

KW - SULFATED GLYCOSAMINOGLYCANS

KW - EXTRACELLULAR-MATRIX

KW - IN-VITRO

KW - HYDROXYPROLINE

KW - RECONSTRUCTION

KW - AUGMENTATION

KW - MODEL

U2 - 10.1016/j.biomaterials.2006.10.005

DO - 10.1016/j.biomaterials.2006.10.005

M3 - Article

C2 - 17092557

SN - 0142-9612

VL - 28

SP - 1061

EP - 1070

JO - Biomaterials

JF - Biomaterials

IS - 6

ER -