DiCre-Based Inducible Gene Expression

Jeziel D Damasceno, Luiz R O Tosi, Renato E R S Santos, Jeremy Charles Mottram

Research output: Chapter in Book/Report/Conference proceedingChapter


Induction of gene expression is a valuable approach for functional studies since it allows for the assessment of phenotypes without the need for clonal selection. Inducible expression can find a wide range of applications, from the study of essential genes to the characterization of overexpression of genes of interest. Here, we describe a detailed protocol for the use of the DiCre-based inducible gene expression system in Leishmania parasites. This is a tightly regulated induction system that allows for time- and dose-controlled expression of gene products, as rapidly as within 12 h.
Original languageEnglish
Title of host publicationLeishmania Methods and Protocols
Number of pages11
ISBN (Electronic)978-1-4939-9210-2
Publication statusPublished - 13 Apr 2019

Publication series

NameMethods in Molecular Biology

Cite this