DiCre-Based Inducible Gene Expression

Jeziel D Damasceno; Luiz R O Tosi; Renato E R S Santos; Jeremy Charles Mottram

doi:10.1007/978-1-4939-9210-2_11

DiCre-Based Inducible Gene Expression

Jeziel D Damasceno, Luiz R O Tosi, Renato E R S Santos, Jeremy Charles Mottram

Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Induction of gene expression is a valuable approach for functional studies since it allows for the assessment of phenotypes without the need for clonal selection. Inducible expression can find a wide range of applications, from the study of essential genes to the characterization of overexpression of genes of interest. Here, we describe a detailed protocol for the use of the DiCre-based inducible gene expression system in Leishmania parasites. This is a tightly regulated induction system that allows for time- and dose-controlled expression of gene products, as rapidly as within 12 h.

Original language	English
Title of host publication	Leishmania Methods and Protocols
Pages	225-235
Number of pages	11
ISBN (Electronic)	978-1-4939-9210-2
DOIs	https://doi.org/10.1007/978-1-4939-9210-2_11
Publication status	Published - 13 Apr 2019

Publication series

Name	Methods in Molecular Biology
Volume	1971

Access to Document

10.1007/978-1-4939-9210-2_11

Cite this

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