By the same authors

DiCre-Based Inducible Gene Expression

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Publication details

Title of host publicationLeishmania Methods and Protocols
DatePublished - 13 Apr 2019
Pages225-235
Number of pages11
Original languageEnglish
ISBN (Electronic)978-1-4939-9210-2

Publication series

NameMethods in Molecular Biology
Volume1971

Abstract

Induction of gene expression is a valuable approach for functional studies since it allows for the assessment of phenotypes without the need for clonal selection. Inducible expression can find a wide range of applications, from the study of essential genes to the characterization of overexpression of genes of interest. Here, we describe a detailed protocol for the use of the DiCre-based inducible gene expression system in Leishmania parasites. This is a tightly regulated induction system that allows for time- and dose-controlled expression of gene products, as rapidly as within 12 h.

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