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From the same journal

Differences In serological IgA responses to recombinant baculovirus-derived human papillomavirus E2 protein in the natural history of cervical neoplasia

Research output: Contribution to journalArticle

Author(s)

  • L RochaZavaleta
  • D Jordan
  • S Pepper
  • G Corbitt
  • F Clarke
  • N J Maitland
  • C M Sanders
  • J R Arrand
  • P L Stern
  • S N Stacey

Department/unit(s)

Publication details

JournalBritish journal of cancer
DatePublished - Apr 1997
Issue number8
Volume75
Number of pages7
Pages (from-to)1144-1150
Original languageEnglish

Abstract

Infection with certain types of human papillomavirus (HPV) presents a high risk for me subsequent development ai cervical intraepithelial neoplasia (GIN) and cervical carcinoma. Immunological mechanisms are likely to play a role in control of cervical HPV lesions. The HPV E2 protein has roles in virus replication and transcription, and loss of E2 functions may be associated with progression of cervical neoplasia. Accordingly, it is of interest to monitor immune responses to the E2 protein, and previous studies have reported associations between serological reactivity to E2 peptide antigens and cervical neoplasia. In order to investigate serological responses to native, full-length E2 protein, we expressed HPV-16 E2 proteins with and without an N-terminal polyhistidine tag using the baculovirus system. Purified HPV-16 E2 protein was used to develop enzyme-linked immunosorbent assays to detect serological IgG and IgA responses in cervical neoplasia patients and controls. We found that serum IgA levels against the E2 protein were elevated in CIN patients relative to normal control subjects but were not elevated in cervical cancer patients. Moreover, there appeared to be a gradient of response within cervical neoplasia such that the highest antibody levels were seen in lower grades of neoplasia up to CIN a, whereas lower levels were observed in CIN 3 and still lower levels in cervical carcinoma. These findings suggest that the IgA antibody response to E2 may associate with stage and progression in cervical neoplasia.

    Research areas

  • human papillomavirus, serology, E2 protein, cervical neoplasia, baculovirus, SERUM ANTIBODY-RESPONSES, CONFORMATIONAL EPITOPES, ANTIPEPTIDE ANTIBODIES, E7 ONCOPROTEINS, CANCER PATIENTS, TYPE-16 E6, HPV-16 E6, INFECTION, DNA, CARCINOMA

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