Differential Dynamic Microscopy: a High-Throughput Method for Characterizing the Motility of Microorganisms

TY - JOUR

T1 - Differential Dynamic Microscopy

T2 - a High-Throughput Method for Characterizing the Motility of Microorganisms

AU - Martinez, Vincent A

AU - Besseling, Rut

AU - Croze, Ottavio A

AU - Tailleur, Julien

AU - Reufer, Mathias

AU - Schwarz-Linek, Jana

AU - Wilson, Laurence G

AU - Bees, Martin A

AU - Poon, Wilson C K

N1 - Copyright © 2012 Biophysical Society. Published by Elsevier Inc. All rights reserved.

PY - 2012/10/17

Y1 - 2012/10/17

N2 - We present a fast, high-throughput method for characterizing the motility of microorganisms in three dimensions based on standard imaging microscopy. Instead of tracking individual cells, we analyze the spatiotemporal fluctuations of the intensity in the sample from time-lapse images and obtain the intermediate scattering function of the system. We demonstrate our method on two different types of microorganisms: the bacterium Escherichia coli (both smooth swimming and wild type) and the biflagellate alga Chlamydomonas reinhardtii. We validate the methodology using computer simulations and particle tracking. From the intermediate scattering function, we are able to extract the swimming speed distribution, fraction of motile cells, and diffusivity for E. coli, and the swimming speed distribution, and amplitude and frequency of the oscillatory dynamics for C. reinhardtii. In both cases, the motility parameters were averaged over ∼10(4) cells and obtained in a few minutes.

AB - We present a fast, high-throughput method for characterizing the motility of microorganisms in three dimensions based on standard imaging microscopy. Instead of tracking individual cells, we analyze the spatiotemporal fluctuations of the intensity in the sample from time-lapse images and obtain the intermediate scattering function of the system. We demonstrate our method on two different types of microorganisms: the bacterium Escherichia coli (both smooth swimming and wild type) and the biflagellate alga Chlamydomonas reinhardtii. We validate the methodology using computer simulations and particle tracking. From the intermediate scattering function, we are able to extract the swimming speed distribution, fraction of motile cells, and diffusivity for E. coli, and the swimming speed distribution, and amplitude and frequency of the oscillatory dynamics for C. reinhardtii. In both cases, the motility parameters were averaged over ∼10(4) cells and obtained in a few minutes.

KW - Cell Tracking

KW - Chlamydomonas reinhardtii

KW - Escherichia coli

KW - Light

KW - Locomotion

KW - Microscopy

KW - Scattering, Radiation

UR - http://www.scopus.com/inward/record.url?scp=84867632858&partnerID=8YFLogxK

U2 - 10.1016/j.bpj.2012.08.045

DO - 10.1016/j.bpj.2012.08.045

M3 - Article

C2 - 23083706

SN - 0006-3495

VL - 103

SP - 1637

EP - 1647

JO - Biophysical Journal

JF - Biophysical Journal

IS - 8

ER -