Direct removal of RNA polymerase barriers to replication by accessory replicative helicases

TY - JOUR

T1 - Direct removal of RNA polymerase barriers to replication by accessory replicative helicases

AU - Hawkins, Michelle Sarah

AU - Dimude, Juachi U.

AU - Howard, Jamieson Anthony Leyland

AU - Smith, Abigail J.

AU - Dillingham, Mark S.

AU - Savery, Nigel J.

AU - Rudolph, Christian J.

AU - McGlynn, Peter

N1 - © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

PY - 2019/6/4

Y1 - 2019/6/4

N2 - Bacterial genome duplication and transcription require simultaneous access to the same DNA template. Conflicts between the replisome and transcription machinery can lead to interruption of DNA replication and loss of genome stability. Pausing, stalling and backtracking of transcribing RNA polymerases add to this problem and present barriers to replisomes. Accessory helicases promote fork movement through nucleoprotein barriers and exist in viruses, bacteria and eukaryotes. Here, we show that stalled Escherichia coli transcription elongation complexes block reconstituted replisomes. This physiologically relevant block can be alleviated by the accessory helicase Rep or UvrD, resulting in the formation of full-length replication products. Accessory helicase action during replication-transcription collisions therefore promotes continued replication without leaving gaps in the DNA. In contrast, DinG does not promote replisome movement through stalled transcription complexes in vitro. However, our data demonstrate that DinG operates indirectly in vivo to reduce conflicts between replication and transcription. These results suggest that Rep and UvrD helicases operate on DNA at the replication fork whereas DinG helicase acts via a different mechanism.

AB - Bacterial genome duplication and transcription require simultaneous access to the same DNA template. Conflicts between the replisome and transcription machinery can lead to interruption of DNA replication and loss of genome stability. Pausing, stalling and backtracking of transcribing RNA polymerases add to this problem and present barriers to replisomes. Accessory helicases promote fork movement through nucleoprotein barriers and exist in viruses, bacteria and eukaryotes. Here, we show that stalled Escherichia coli transcription elongation complexes block reconstituted replisomes. This physiologically relevant block can be alleviated by the accessory helicase Rep or UvrD, resulting in the formation of full-length replication products. Accessory helicase action during replication-transcription collisions therefore promotes continued replication without leaving gaps in the DNA. In contrast, DinG does not promote replisome movement through stalled transcription complexes in vitro. However, our data demonstrate that DinG operates indirectly in vivo to reduce conflicts between replication and transcription. These results suggest that Rep and UvrD helicases operate on DNA at the replication fork whereas DinG helicase acts via a different mechanism.

UR - http://www.scopus.com/inward/record.url?scp=85067201606&partnerID=8YFLogxK

U2 - 10.1093/nar/gkz170

DO - 10.1093/nar/gkz170

M3 - Article

C2 - 30869136

SN - 0305-1048

VL - 47

SP - 5100

EP - 5113

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 10

ER -