Dishevelled (Dvl-2) activates canonical Wnt signalling in the absence of cytoplasmic puncta

M.J. Smalley; N. Signoret; D. Robertson; A. Tilley; A. Hann; K. Ewan; Y. Ding; H. Paterson; T.C. Dale

doi:10.1242/jcs.02647

Dishevelled (Dvl-2) activates canonical Wnt signalling in the absence of cytoplasmic puncta

M.J. Smalley, N. Signoret, D. Robertson, A. Tilley, A. Hann, K. Ewan, Y. Ding, H. Paterson, T.C. Dale

The Hull York Medical School

Research output: Contribution to journal › Article › peer-review

Abstract

Dishevelled family proteins are multidomain intracellular transducers of Wnt signals. Ectopically expressed mammalian Dishevelled 2 (Dvl-2) activates downstream signalling and localises to cytoplasmic puncta. It has been suggested that these Dvl-2-containing structures correspond to intracellular vesicles and may be involved in the Wnt signal transduction process. We report that cytoplasmic puncta are primarily formed in cells expressing Dvl-2 at high levels. Lower levels of expression can activate signalling without forming puncta. The structures do not localise with markers of the early or late endocytic pathway and time-lapse analysis demonstrates that Dvl-2 puncta move in a random fashion over short distances but do not originate from the plasma membrane. Based on our findings, we propose that Dvl-2 puncta are protein aggregates that are not required for signalling.

Original language	English
Pages (from-to)	5279-5289
Number of pages	10
Journal	Journal of Cell Science
Volume	118
Issue number	22
DOIs	https://doi.org/10.1242/jcs.02647
Publication status	Published - Nov 2005

Access to Document

10.1242/jcs.02647

http://dx.doi.org/10.1242/jcs.02647

Cite this

@article{84078afea8284043a6b7a8dcaaaa06ad,

title = "Dishevelled (Dvl-2) activates canonical Wnt signalling in the absence of cytoplasmic puncta",

abstract = "Dishevelled family proteins are multidomain intracellular transducers of Wnt signals. Ectopically expressed mammalian Dishevelled 2 (Dvl-2) activates downstream signalling and localises to cytoplasmic puncta. It has been suggested that these Dvl-2-containing structures correspond to intracellular vesicles and may be involved in the Wnt signal transduction process. We report that cytoplasmic puncta are primarily formed in cells expressing Dvl-2 at high levels. Lower levels of expression can activate signalling without forming puncta. The structures do not localise with markers of the early or late endocytic pathway and time-lapse analysis demonstrates that Dvl-2 puncta move in a random fashion over short distances but do not originate from the plasma membrane. Based on our findings, we propose that Dvl-2 puncta are protein aggregates that are not required for signalling.",

author = "M.J. Smalley and N. Signoret and D. Robertson and A. Tilley and A. Hann and K. Ewan and Y. Ding and H. Paterson and T.C. Dale",

year = "2005",

month = nov,

doi = "10.1242/jcs.02647",

language = "English",

volume = "118",

pages = "5279--5289",

journal = "Journal of Cell Science",

issn = "0021-9533",

publisher = "Company of Biologists Ltd",

number = "22",

}

TY - JOUR

T1 - Dishevelled (Dvl-2) activates canonical Wnt signalling in the absence of cytoplasmic puncta

AU - Smalley, M.J.

AU - Signoret, N.

AU - Robertson, D.

AU - Tilley, A.

AU - Hann, A.

AU - Ewan, K.

AU - Ding, Y.

AU - Paterson, H.

AU - Dale, T.C.

PY - 2005/11

Y1 - 2005/11

N2 - Dishevelled family proteins are multidomain intracellular transducers of Wnt signals. Ectopically expressed mammalian Dishevelled 2 (Dvl-2) activates downstream signalling and localises to cytoplasmic puncta. It has been suggested that these Dvl-2-containing structures correspond to intracellular vesicles and may be involved in the Wnt signal transduction process. We report that cytoplasmic puncta are primarily formed in cells expressing Dvl-2 at high levels. Lower levels of expression can activate signalling without forming puncta. The structures do not localise with markers of the early or late endocytic pathway and time-lapse analysis demonstrates that Dvl-2 puncta move in a random fashion over short distances but do not originate from the plasma membrane. Based on our findings, we propose that Dvl-2 puncta are protein aggregates that are not required for signalling.

AB - Dishevelled family proteins are multidomain intracellular transducers of Wnt signals. Ectopically expressed mammalian Dishevelled 2 (Dvl-2) activates downstream signalling and localises to cytoplasmic puncta. It has been suggested that these Dvl-2-containing structures correspond to intracellular vesicles and may be involved in the Wnt signal transduction process. We report that cytoplasmic puncta are primarily formed in cells expressing Dvl-2 at high levels. Lower levels of expression can activate signalling without forming puncta. The structures do not localise with markers of the early or late endocytic pathway and time-lapse analysis demonstrates that Dvl-2 puncta move in a random fashion over short distances but do not originate from the plasma membrane. Based on our findings, we propose that Dvl-2 puncta are protein aggregates that are not required for signalling.

U2 - 10.1242/jcs.02647

DO - 10.1242/jcs.02647

M3 - Article

SN - 0021-9533

VL - 118

SP - 5279

EP - 5289

JO - Journal of Cell Science

JF - Journal of Cell Science

IS - 22

ER -