TY - JOUR
T1 - Disruption of a DUF247 Containing Protein Alters Cell Wall Polysaccharides and Reduces Growth in Arabidopsis
AU - Wannitikul, Pitchaporn
AU - Wattana-Amorn, Pakorn
AU - Sathitnaitham, Sukhita
AU - Sakulkoo, Jenjira
AU - Suttangkakul, Anongpat
AU - Wonnapinij, Passorn
AU - Bassel, George W
AU - Simister, Rachael
AU - Gomez, Leonardo D
AU - Vuttipongchaikij, Supachai
N1 - © 2023 by the authors
PY - 2023/5/15
Y1 - 2023/5/15
N2 - Plant cell wall biosynthesis is a complex process that requires proteins and enzymes from glycan synthesis to wall assembly. We show that disruption of
At3g50120 (
DUF247-1), a member of the
DUF247 multigene family containing 28 genes in
Arabidopsis, results in alterations to the structure and composition of cell wall polysaccharides and reduced growth and plant size. An ELISA using cell wall antibodies shows that the mutants also exhibit ~50% reductions in xyloglucan (XyG), glucuronoxylan (GX) and heteromannan (HM) epitopes in the NaOH fraction and ~50% increases in homogalacturonan (HG) epitopes in the CDTA fraction. Furthermore, the polymer sizes of XyGs and GXs are reduced with concomitant increases in short-chain polymers, while those of HGs and mHGs are slightly increased. Complementation using 35S:
DUF247-1 partially recovers the XyG and HG content, but not those of GX and HM, suggesting that DUF247-1 is more closely associated with XyGs and HGs.
DUF247-1 is expressed throughout
Arabidopsis, particularly in vascular and developing tissues, and its disruption affects the expression of other gene members, indicating a regulatory control role within the gene family. Our results demonstrate that DUF247-1 is required for normal cell wall composition and structure and
Arabidopsis growth.
AB - Plant cell wall biosynthesis is a complex process that requires proteins and enzymes from glycan synthesis to wall assembly. We show that disruption of
At3g50120 (
DUF247-1), a member of the
DUF247 multigene family containing 28 genes in
Arabidopsis, results in alterations to the structure and composition of cell wall polysaccharides and reduced growth and plant size. An ELISA using cell wall antibodies shows that the mutants also exhibit ~50% reductions in xyloglucan (XyG), glucuronoxylan (GX) and heteromannan (HM) epitopes in the NaOH fraction and ~50% increases in homogalacturonan (HG) epitopes in the CDTA fraction. Furthermore, the polymer sizes of XyGs and GXs are reduced with concomitant increases in short-chain polymers, while those of HGs and mHGs are slightly increased. Complementation using 35S:
DUF247-1 partially recovers the XyG and HG content, but not those of GX and HM, suggesting that DUF247-1 is more closely associated with XyGs and HGs.
DUF247-1 is expressed throughout
Arabidopsis, particularly in vascular and developing tissues, and its disruption affects the expression of other gene members, indicating a regulatory control role within the gene family. Our results demonstrate that DUF247-1 is required for normal cell wall composition and structure and
Arabidopsis growth.
U2 - 10.3390/plants12101977
DO - 10.3390/plants12101977
M3 - Article
C2 - 37653894
SN - 2223-7747
VL - 12
JO - Plants
JF - Plants
IS - 10
M1 - 1977
ER -