Dissecting biomethane production in anaerobic digestion plants through DNA sequencing

Research output: Contribution to conferencePosterpeer-review

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Dissecting biomethane production in anaerobic digestion plants through DNA sequencing. / Alessi, Anna; Tao, Bing; Heaven, Sonia; Banks, Charles; Thomas-Oates, Jane Elizabeth; Chong, James Paul Jonathan.

2018. Poster session presented at Microbiology Society Annual Conferene, Birmingham, United Kingdom.

Research output: Contribution to conferencePosterpeer-review

Harvard

Alessi, A, Tao, B, Heaven, S, Banks, C, Thomas-Oates, JE & Chong, JPJ 2018, 'Dissecting biomethane production in anaerobic digestion plants through DNA sequencing', Microbiology Society Annual Conferene, Birmingham, United Kingdom, 10/04/18 - 13/04/18.

APA

Alessi, A., Tao, B., Heaven, S., Banks, C., Thomas-Oates, J. E., & Chong, J. P. J. (2018). Dissecting biomethane production in anaerobic digestion plants through DNA sequencing. Poster session presented at Microbiology Society Annual Conferene, Birmingham, United Kingdom.

Vancouver

Alessi A, Tao B, Heaven S, Banks C, Thomas-Oates JE, Chong JPJ. Dissecting biomethane production in anaerobic digestion plants through DNA sequencing. 2018. Poster session presented at Microbiology Society Annual Conferene, Birmingham, United Kingdom.

Author

Alessi, Anna ; Tao, Bing ; Heaven, Sonia ; Banks, Charles ; Thomas-Oates, Jane Elizabeth ; Chong, James Paul Jonathan. / Dissecting biomethane production in anaerobic digestion plants through DNA sequencing. Poster session presented at Microbiology Society Annual Conferene, Birmingham, United Kingdom.

Bibtex - Download

@conference{1cee757c0cfc4faa856fe4f9a33fbc34,
title = "Dissecting biomethane production in anaerobic digestion plants through DNA sequencing",
abstract = "Production of methane via biologically optimised process offer an alternative to natural gas consumption as its demand increases annually (EU by 7.1% since 2010). Processes of converting domestic waste to gaseous, energy-rich biomethane are reported across the world with the potential to use this renewable energy source for national grid balancing and transportation. Biomethane production however requires upgrading which is a cost-limiting step making the product economically unviable. With experimental approaches of laboratory scale reactors and DNA sequencing, we studied the identity and physiology of the microorganisms responsible for anaerobic digestion and methane production. Reactors{\textquoteright} performance was monitored daily for one year to collect robust operational data that will be used for scaling the reactors{\textquoteright} working volume and for predicting their performance at industrial scale. Biological samples were used for DNA metagenome sequencing. By combining short and long reads sequencing techniques, it was possible to obtain the complete or nearly complete genomes for several of the most abundant microorganisms. The DNA sequences revealed that some of these microorganisms are able to utilise sugars to produce hydrogen, CO2 and acids while others are efficient in converting these compounds as substrates to a high quality biomethane. This provides evidence that close interactions between microorganisms occur in the bioreactors, occupied by highly specialized and enriched community. The sequencing results enable a deeper understanding of the diversity and ecology of the resident microbial community that in the future could be manipulated to increase the efficiency and quality of the anaerobic digestion process. ",
author = "Anna Alessi and Bing Tao and Sonia Heaven and Charles Banks and Thomas-Oates, {Jane Elizabeth} and Chong, {James Paul Jonathan}",
year = "2018",
month = apr,
day = "11",
language = "English",
note = "Microbiology Society Annual Conferene ; Conference date: 10-04-2018 Through 13-04-2018",

}

RIS (suitable for import to EndNote) - Download

TY - CONF

T1 - Dissecting biomethane production in anaerobic digestion plants through DNA sequencing

AU - Alessi, Anna

AU - Tao, Bing

AU - Heaven, Sonia

AU - Banks, Charles

AU - Thomas-Oates, Jane Elizabeth

AU - Chong, James Paul Jonathan

PY - 2018/4/11

Y1 - 2018/4/11

N2 - Production of methane via biologically optimised process offer an alternative to natural gas consumption as its demand increases annually (EU by 7.1% since 2010). Processes of converting domestic waste to gaseous, energy-rich biomethane are reported across the world with the potential to use this renewable energy source for national grid balancing and transportation. Biomethane production however requires upgrading which is a cost-limiting step making the product economically unviable. With experimental approaches of laboratory scale reactors and DNA sequencing, we studied the identity and physiology of the microorganisms responsible for anaerobic digestion and methane production. Reactors’ performance was monitored daily for one year to collect robust operational data that will be used for scaling the reactors’ working volume and for predicting their performance at industrial scale. Biological samples were used for DNA metagenome sequencing. By combining short and long reads sequencing techniques, it was possible to obtain the complete or nearly complete genomes for several of the most abundant microorganisms. The DNA sequences revealed that some of these microorganisms are able to utilise sugars to produce hydrogen, CO2 and acids while others are efficient in converting these compounds as substrates to a high quality biomethane. This provides evidence that close interactions between microorganisms occur in the bioreactors, occupied by highly specialized and enriched community. The sequencing results enable a deeper understanding of the diversity and ecology of the resident microbial community that in the future could be manipulated to increase the efficiency and quality of the anaerobic digestion process.

AB - Production of methane via biologically optimised process offer an alternative to natural gas consumption as its demand increases annually (EU by 7.1% since 2010). Processes of converting domestic waste to gaseous, energy-rich biomethane are reported across the world with the potential to use this renewable energy source for national grid balancing and transportation. Biomethane production however requires upgrading which is a cost-limiting step making the product economically unviable. With experimental approaches of laboratory scale reactors and DNA sequencing, we studied the identity and physiology of the microorganisms responsible for anaerobic digestion and methane production. Reactors’ performance was monitored daily for one year to collect robust operational data that will be used for scaling the reactors’ working volume and for predicting their performance at industrial scale. Biological samples were used for DNA metagenome sequencing. By combining short and long reads sequencing techniques, it was possible to obtain the complete or nearly complete genomes for several of the most abundant microorganisms. The DNA sequences revealed that some of these microorganisms are able to utilise sugars to produce hydrogen, CO2 and acids while others are efficient in converting these compounds as substrates to a high quality biomethane. This provides evidence that close interactions between microorganisms occur in the bioreactors, occupied by highly specialized and enriched community. The sequencing results enable a deeper understanding of the diversity and ecology of the resident microbial community that in the future could be manipulated to increase the efficiency and quality of the anaerobic digestion process.

M3 - Poster

T2 - Microbiology Society Annual Conferene

Y2 - 10 April 2018 through 13 April 2018

ER -