Dissecting functions of the conserved oligomeric Golgi tethering complex using a cell free assay

TY - JOUR

T1 - Dissecting functions of the conserved oligomeric Golgi tethering complex using a cell free assay

AU - Cottam, Nathanael P

AU - Wilson, Katherine M

AU - Ng, Bobby G

AU - Körner, Christian

AU - Freeze, Hudson H

AU - Ungar, Daniel

N1 - This article is protected by copyright. All rights reserved.

PY - 2014/1/4

Y1 - 2014/1/4

N2 - Vesicle transport sorts proteins between compartments and is thereby responsible for generating the non-uniform protein distribution along the eukaryotic secretory and endocytic pathways. The mechanistic details of specific vesicle targeting are not yet well characterised at the molecular level. We have developed a cell free assay that reconstitutes vesicle targeting utilising the recycling of resident enzymes within the Golgi apparatus. The assay has physiological properties, and could be used to show that the two lobes of the conserved oligomeric Golgi tethering complex play antagonistic roles in trans-Golgi vesicle targeting. Moreover, we can show that the assay is sensitive to several different congenital defects that disrupt Golgi function and therefore cause glycosylation disorders. Consequently, this assay will allow mechanistic insight into the targeting step of vesicle transport at the Golgi, and could also be useful for characterising some novel cases of congenital glycosylation disorders.

AB - Vesicle transport sorts proteins between compartments and is thereby responsible for generating the non-uniform protein distribution along the eukaryotic secretory and endocytic pathways. The mechanistic details of specific vesicle targeting are not yet well characterised at the molecular level. We have developed a cell free assay that reconstitutes vesicle targeting utilising the recycling of resident enzymes within the Golgi apparatus. The assay has physiological properties, and could be used to show that the two lobes of the conserved oligomeric Golgi tethering complex play antagonistic roles in trans-Golgi vesicle targeting. Moreover, we can show that the assay is sensitive to several different congenital defects that disrupt Golgi function and therefore cause glycosylation disorders. Consequently, this assay will allow mechanistic insight into the targeting step of vesicle transport at the Golgi, and could also be useful for characterising some novel cases of congenital glycosylation disorders.

U2 - 10.1111/tra.12128

DO - 10.1111/tra.12128

M3 - Article

C2 - 24102787

SN - 1600-0854

VL - 15

SP - 12

EP - 21

JO - Traffic

JF - Traffic

IS - 1

ER -