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Dissection of control of anion homeostasis by Associative Transcriptomics in Brassica napus

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Dissection of control of anion homeostasis by Associative Transcriptomics in Brassica napus. / Koprivova, Anna; Harper, Andrea L; Trick, Martin; Bancroft, Ian; Kopriva, Stanislav.

In: Plant Physiology, Vol. 166, No. September, 30.08.2014, p. 442-450.

Research output: Contribution to journalArticle

Harvard

Koprivova, A, Harper, AL, Trick, M, Bancroft, I & Kopriva, S 2014, 'Dissection of control of anion homeostasis by Associative Transcriptomics in Brassica napus', Plant Physiology, vol. 166, no. September, pp. 442-450. https://doi.org/10.1104/pp.114.239947

APA

Koprivova, A., Harper, A. L., Trick, M., Bancroft, I., & Kopriva, S. (2014). Dissection of control of anion homeostasis by Associative Transcriptomics in Brassica napus. Plant Physiology, 166(September), 442-450. https://doi.org/10.1104/pp.114.239947

Vancouver

Koprivova A, Harper AL, Trick M, Bancroft I, Kopriva S. Dissection of control of anion homeostasis by Associative Transcriptomics in Brassica napus. Plant Physiology. 2014 Aug 30;166(September):442-450. https://doi.org/10.1104/pp.114.239947

Author

Koprivova, Anna ; Harper, Andrea L ; Trick, Martin ; Bancroft, Ian ; Kopriva, Stanislav. / Dissection of control of anion homeostasis by Associative Transcriptomics in Brassica napus. In: Plant Physiology. 2014 ; Vol. 166, No. September. pp. 442-450.

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@article{99e9f0dd7a0a479abc765f68c337340d,
title = "Dissection of control of anion homeostasis by Associative Transcriptomics in Brassica napus",
abstract = "To assess the variation in nutrient homeostasis in oilseed rape and to identify genes responsible for this variation we determined foliar anion levels in a diversity panel of Brassica napus accessions, 84 of which had been genotyped previously using mRNA sequencing. We applied Associative Transcriptomics (AT) to identify sequence polymorphisms linked to variation in nitrate, phosphate, or sulfate in these accessions. The analysis identified several hundred significant associations for each anion. Using functional annotation of Arabidopsis homologues and available microarray data we identified 60 candidate genes for controlling variation in the anion contents. To verify that these genes function in control of nutrient homeostasis we obtained Arabidopsis T-DNA insertion lines for these candidates and tested them for accumulation of nitrate, phosphate, and sulfate. Fourteen lines differed significantly in levels of the corresponding anions. Several of these genes have been shown previously to affect accumulation of the corresponding anions in Arabidopsis mutants. These results thus confirm the power of AT in dissection of the genetic control of complex traits and present a set of candidate genes for use in improvement of efficiency of B. napus mineral nutrition.",
author = "Anna Koprivova and Harper, {Andrea L} and Martin Trick and Ian Bancroft and Stanislav Kopriva",
note = "Copyright {\circledC} 2014, American Society of Plant Biologists.",
year = "2014",
month = "8",
day = "30",
doi = "10.1104/pp.114.239947",
language = "English",
volume = "166",
pages = "442--450",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "September",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Dissection of control of anion homeostasis by Associative Transcriptomics in Brassica napus

AU - Koprivova, Anna

AU - Harper, Andrea L

AU - Trick, Martin

AU - Bancroft, Ian

AU - Kopriva, Stanislav

N1 - Copyright © 2014, American Society of Plant Biologists.

PY - 2014/8/30

Y1 - 2014/8/30

N2 - To assess the variation in nutrient homeostasis in oilseed rape and to identify genes responsible for this variation we determined foliar anion levels in a diversity panel of Brassica napus accessions, 84 of which had been genotyped previously using mRNA sequencing. We applied Associative Transcriptomics (AT) to identify sequence polymorphisms linked to variation in nitrate, phosphate, or sulfate in these accessions. The analysis identified several hundred significant associations for each anion. Using functional annotation of Arabidopsis homologues and available microarray data we identified 60 candidate genes for controlling variation in the anion contents. To verify that these genes function in control of nutrient homeostasis we obtained Arabidopsis T-DNA insertion lines for these candidates and tested them for accumulation of nitrate, phosphate, and sulfate. Fourteen lines differed significantly in levels of the corresponding anions. Several of these genes have been shown previously to affect accumulation of the corresponding anions in Arabidopsis mutants. These results thus confirm the power of AT in dissection of the genetic control of complex traits and present a set of candidate genes for use in improvement of efficiency of B. napus mineral nutrition.

AB - To assess the variation in nutrient homeostasis in oilseed rape and to identify genes responsible for this variation we determined foliar anion levels in a diversity panel of Brassica napus accessions, 84 of which had been genotyped previously using mRNA sequencing. We applied Associative Transcriptomics (AT) to identify sequence polymorphisms linked to variation in nitrate, phosphate, or sulfate in these accessions. The analysis identified several hundred significant associations for each anion. Using functional annotation of Arabidopsis homologues and available microarray data we identified 60 candidate genes for controlling variation in the anion contents. To verify that these genes function in control of nutrient homeostasis we obtained Arabidopsis T-DNA insertion lines for these candidates and tested them for accumulation of nitrate, phosphate, and sulfate. Fourteen lines differed significantly in levels of the corresponding anions. Several of these genes have been shown previously to affect accumulation of the corresponding anions in Arabidopsis mutants. These results thus confirm the power of AT in dissection of the genetic control of complex traits and present a set of candidate genes for use in improvement of efficiency of B. napus mineral nutrition.

U2 - 10.1104/pp.114.239947

DO - 10.1104/pp.114.239947

M3 - Article

VL - 166

SP - 442

EP - 450

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - September

ER -