DNA content and nucleoid distribution in Methanothermobacter thermautotrophicus

A I Majernik; M Lundgren; P McDermott; R Bernander; J P J Chong

doi:10.1128/JB.187.5.1856-1858.2005

DNA content and nucleoid distribution in Methanothermobacter thermautotrophicus

A I Majernik, M Lundgren, P McDermott, R Bernander, J P J Chong

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Flow cytometry and epifluorescence microscopy results for the euryarchaeon Methanothermobacter thermautotrophicus were consistent with filaments containing multiple cells. Filaments of one to four cells contained two to eight nucleoids. Single chromosome-containing cells were not observed. Filaments containing multiple genome copies displayed synchronous DNA replication initiation. Chromosome segregation occurred during replication or rapidly after replication termination.

Original language	English
Pages (from-to)	1856-1858
Number of pages	3
Journal	Journal of Bacteriology
Volume	187
Issue number	5
DOIs	https://doi.org/10.1128/JB.187.5.1856-1858.2005
Publication status	Published - Mar 2005

Keywords

MINICHROMOSOME MAINTENANCE PROTEIN
THERMOAUTOTROPHICUM DELTA-H
COMPLETE GENOME SEQUENCE
METHANOBACTERIUM-THERMOAUTOTROPHICUM
ESCHERICHIA-COLI
CHROMOSOME-REPLICATION
THERMOPHILIC ARCHAEA
CELL-CYCLE
HELICASE
MCM

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10.1128/JB.187.5.1856-1858.2005

http://dx.doi.org/10.1128/JB.187.5.1856-1858.2005

Characterisation of DNA Replication in Archaea
Chong, J.
BBSRC
1/01/04 → 28/08/05
Project: Research project (funded) › Research

Cite this

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title = "DNA content and nucleoid distribution in Methanothermobacter thermautotrophicus",

abstract = "Flow cytometry and epifluorescence microscopy results for the euryarchaeon Methanothermobacter thermautotrophicus were consistent with filaments containing multiple cells. Filaments of one to four cells contained two to eight nucleoids. Single chromosome-containing cells were not observed. Filaments containing multiple genome copies displayed synchronous DNA replication initiation. Chromosome segregation occurred during replication or rapidly after replication termination.",

keywords = "MINICHROMOSOME MAINTENANCE PROTEIN, THERMOAUTOTROPHICUM DELTA-H, COMPLETE GENOME SEQUENCE, METHANOBACTERIUM-THERMOAUTOTROPHICUM, ESCHERICHIA-COLI, CHROMOSOME-REPLICATION, THERMOPHILIC ARCHAEA, CELL-CYCLE, HELICASE, MCM",

author = "Majernik, {A I} and M Lundgren and P McDermott and R Bernander and Chong, {J P J}",

year = "2005",

month = mar,

doi = "10.1128/JB.187.5.1856-1858.2005",

language = "English",

volume = "187",

pages = "1856--1858",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "5",

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TY - JOUR

T1 - DNA content and nucleoid distribution in Methanothermobacter thermautotrophicus

AU - Majernik, A I

AU - Lundgren, M

AU - McDermott, P

AU - Bernander, R

AU - Chong, J P J

PY - 2005/3

Y1 - 2005/3

N2 - Flow cytometry and epifluorescence microscopy results for the euryarchaeon Methanothermobacter thermautotrophicus were consistent with filaments containing multiple cells. Filaments of one to four cells contained two to eight nucleoids. Single chromosome-containing cells were not observed. Filaments containing multiple genome copies displayed synchronous DNA replication initiation. Chromosome segregation occurred during replication or rapidly after replication termination.

AB - Flow cytometry and epifluorescence microscopy results for the euryarchaeon Methanothermobacter thermautotrophicus were consistent with filaments containing multiple cells. Filaments of one to four cells contained two to eight nucleoids. Single chromosome-containing cells were not observed. Filaments containing multiple genome copies displayed synchronous DNA replication initiation. Chromosome segregation occurred during replication or rapidly after replication termination.

KW - MINICHROMOSOME MAINTENANCE PROTEIN

KW - THERMOAUTOTROPHICUM DELTA-H

KW - COMPLETE GENOME SEQUENCE

KW - METHANOBACTERIUM-THERMOAUTOTROPHICUM

KW - ESCHERICHIA-COLI

KW - CHROMOSOME-REPLICATION

KW - THERMOPHILIC ARCHAEA

KW - CELL-CYCLE

KW - HELICASE

KW - MCM

U2 - 10.1128/JB.187.5.1856-1858.2005

DO - 10.1128/JB.187.5.1856-1858.2005

M3 - Article

SN - 0021-9193

VL - 187

SP - 1856

EP - 1858

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 5

ER -

DNA content and nucleoid distribution in Methanothermobacter thermautotrophicus

Abstract

Keywords

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Projects

Characterisation of DNA Replication in Archaea

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