Abstract
EPR spectroscopy provides direct evidence for the intercalation of copper(II) complexes of 1,10-phenanthroline and 2,2'-bipyridine with DNA. Reduction of these complexes to copper(I) by glutathione is evidently facile, whereas ascorbate reacts more slowly. Reoxidation of copper(I) with (BuOOH)-Bu-t (to give (BuO.)-Bu-t) is rapid in both systems, as judged by EPR spin-trapping results. Reaction of copper(I)-DNA with H2O2 in the presence of ascorbate leads to the generation of HO. and to the trapping of DNA-derived radical adducts. The role of 1,10-phenanthroline and ascorbate, especially, and the relevance to DNA scission, are discussed.
Original language | English |
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Pages (from-to) | 1891-1895 |
Number of pages | 5 |
Journal | JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2 |
Issue number | 9 |
Publication status | Published - 1999 |
Keywords
- ELECTRON-SPIN RESONANCE
- STRAND SCISSION
- GLUTATHIONE
- OXIDATION
- BINDING