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DNA damage via intercalation of copper complexes and activation by ascorbate and peroxides: direct EPR evidence for hydroxyl radical formation and reaction

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JournalJOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2
DatePublished - 1999
Issue number9
Number of pages5
Pages (from-to)1891-1895
Original languageEnglish

Abstract

EPR spectroscopy provides direct evidence for the intercalation of copper(II) complexes of 1,10-phenanthroline and 2,2'-bipyridine with DNA. Reduction of these complexes to copper(I) by glutathione is evidently facile, whereas ascorbate reacts more slowly. Reoxidation of copper(I) with (BuOOH)-Bu-t (to give (BuO.)-Bu-t) is rapid in both systems, as judged by EPR spin-trapping results. Reaction of copper(I)-DNA with H2O2 in the presence of ascorbate leads to the generation of HO. and to the trapping of DNA-derived radical adducts. The role of 1,10-phenanthroline and ascorbate, especially, and the relevance to DNA scission, are discussed.

    Research areas

  • ELECTRON-SPIN RESONANCE, STRAND SCISSION, GLUTATHIONE, OXIDATION, BINDING

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