DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase.

Tom Killelea; Michelle Sarah Hawkins; Jamieson Anthony Leyland Howard; Peter McGlynn; Edward L. Bolt

doi:10.1080/15476286.2018.1496773

DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase.

Tom Killelea, Michelle Sarah Hawkins, Jamieson Anthony Leyland Howard, Peter McGlynn, Edward L. Bolt

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Cascade complexes underpin E. coli CRISPR-Cas immunity systems by stimulating "adaptation" reactions that update immunity and by initiating "interference" reactions that destroy invader DNA. Recognition of invader DNA in Cascade catalysed R-loops provokes DNA capture and its subsequent integration into CRISPR loci by Cas1 and Cas2. DNA capture processes are unclear but may involve RecG helicase, which stimulates adaptation during its role responding to genome instability. We show that Cascade is a potential source of genome instability because it blocks DNA replication and that RecG helicase alleviates this by dissociating Cascade. This highlights how integrating in vitro CRISPR-Cas interference and adaptation reactions with DNA replication and repair reactions will help to determine precise mechanisms underpinning prokaryotic adaptive immunity.

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	RNA Biology
Early online date	10 Aug 2018
DOIs	https://doi.org/10.1080/15476286.2018.1496773
Publication status	Published - 10 Aug 2018

Bibliographical note

Access to Document

10.1080/15476286.2018.1496773Licence: CC BY

McGlynn - Accepted Version
© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
Accepted author manuscript, 709 KBLicence: CC BY
DNA replication roadblocks caused by Cascade interference complexes are alleviated by RecG DNA repair helicase
© 2018 The Author(s).
Final published version, 2.14 MBLicence: CC BY

Cite this

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title = "DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase.",

abstract = "Cascade complexes underpin E. coli CRISPR-Cas immunity systems by stimulating {"}adaptation{"} reactions that update immunity and by initiating {"}interference{"} reactions that destroy invader DNA. Recognition of invader DNA in Cascade catalysed R-loops provokes DNA capture and its subsequent integration into CRISPR loci by Cas1 and Cas2. DNA capture processes are unclear but may involve RecG helicase, which stimulates adaptation during its role responding to genome instability. We show that Cascade is a potential source of genome instability because it blocks DNA replication and that RecG helicase alleviates this by dissociating Cascade. This highlights how integrating in vitro CRISPR-Cas interference and adaptation reactions with DNA replication and repair reactions will help to determine precise mechanisms underpinning prokaryotic adaptive immunity.",

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doi = "10.1080/15476286.2018.1496773",

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T1 - DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase.

AU - Killelea, Tom

AU - Hawkins, Michelle Sarah

AU - Howard, Jamieson Anthony Leyland

AU - McGlynn, Peter

AU - Bolt, Edward L.

PY - 2018/8/10

Y1 - 2018/8/10

N2 - Cascade complexes underpin E. coli CRISPR-Cas immunity systems by stimulating "adaptation" reactions that update immunity and by initiating "interference" reactions that destroy invader DNA. Recognition of invader DNA in Cascade catalysed R-loops provokes DNA capture and its subsequent integration into CRISPR loci by Cas1 and Cas2. DNA capture processes are unclear but may involve RecG helicase, which stimulates adaptation during its role responding to genome instability. We show that Cascade is a potential source of genome instability because it blocks DNA replication and that RecG helicase alleviates this by dissociating Cascade. This highlights how integrating in vitro CRISPR-Cas interference and adaptation reactions with DNA replication and repair reactions will help to determine precise mechanisms underpinning prokaryotic adaptive immunity.

AB - Cascade complexes underpin E. coli CRISPR-Cas immunity systems by stimulating "adaptation" reactions that update immunity and by initiating "interference" reactions that destroy invader DNA. Recognition of invader DNA in Cascade catalysed R-loops provokes DNA capture and its subsequent integration into CRISPR loci by Cas1 and Cas2. DNA capture processes are unclear but may involve RecG helicase, which stimulates adaptation during its role responding to genome instability. We show that Cascade is a potential source of genome instability because it blocks DNA replication and that RecG helicase alleviates this by dissociating Cascade. This highlights how integrating in vitro CRISPR-Cas interference and adaptation reactions with DNA replication and repair reactions will help to determine precise mechanisms underpinning prokaryotic adaptive immunity.

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