DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase.

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Publication details

JournalRNA Biology
DateAccepted/In press - 29 Jun 2018
DateE-pub ahead of print - 10 Aug 2018
DatePublished (current) - 10 Aug 2018
Number of pages6
Pages (from-to)1-6
Early online date10/08/18
Original languageEnglish


Cascade complexes underpin E. coli CRISPR-Cas immunity systems by stimulating "adaptation" reactions that update immunity and by initiating "interference" reactions that destroy invader DNA. Recognition of invader DNA in Cascade catalysed R-loops provokes DNA capture and its subsequent integration into CRISPR loci by Cas1 and Cas2. DNA capture processes are unclear but may involve RecG helicase, which stimulates adaptation during its role responding to genome instability. We show that Cascade is a potential source of genome instability because it blocks DNA replication and that RecG helicase alleviates this by dissociating Cascade. This highlights how integrating in vitro CRISPR-Cas interference and adaptation reactions with DNA replication and repair reactions will help to determine precise mechanisms underpinning prokaryotic adaptive immunity.

Bibliographical note

© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

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