DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision

Lars Schomacher; James P. J. Chong; Paul McDermott; Wilfried Kramer; Hans-Joachim Fritz

doi:10.1093/nar/gkp102

DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision

Lars Schomacher, James P. J. Chong, Paul McDermott, Wilfried Kramer, Hans-Joachim Fritz

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

No genes for any of the known uracil DNA glycosylases of the UDG superfamily are present in the genome of Methanothermobacter thermautotrophicus H, making it difficult to imagine how DNA-U repair might be initiated in this organism. Recently, Mth212, the ExoIII homologue of M. thermautotrophicus H has been characterized as a DNA uridine endonuclease, which suggested the possibility of a novel endonucleolytic entry mechanism for DNA uracil repair. With no system of genetic experimentation available, the problem was approached biochemically. Assays of DNA uracil repair in vitro, promoted by crude cellular extracts, provide unequivocal confirmation that this mechanism does indeed operate in M. thermautotrophicus H.

Original language	English
Pages (from-to)	2283-2293
Number of pages	11
Journal	Nucleic Acids Research
Volume	37
Issue number	7
DOIs	https://doi.org/10.1093/nar/gkp102
Publication status	Published - Apr 2009

Keywords

BASE EXCISION-REPAIR
ESCHERICHIA-COLI K-12
THERMOAUTOTROPHICUM DELTA-H
COMPLETE GENOME SEQUENCE
METHANOBACTERIUM-THERMOAUTOTROPHICUM
METHANOTHERMOBACTER-THERMAUTOTROPHICUS
MISMATCH ENDONUCLEASE
EXONUCLEASE-III
POLYMERASE
GENE

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10.1093/nar/gkp102

http://nar.oxfordjournals.org/content/37/7/2283

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title = "DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision",

abstract = "No genes for any of the known uracil DNA glycosylases of the UDG superfamily are present in the genome of Methanothermobacter thermautotrophicus H, making it difficult to imagine how DNA-U repair might be initiated in this organism. Recently, Mth212, the ExoIII homologue of M. thermautotrophicus H has been characterized as a DNA uridine endonuclease, which suggested the possibility of a novel endonucleolytic entry mechanism for DNA uracil repair. With no system of genetic experimentation available, the problem was approached biochemically. Assays of DNA uracil repair in vitro, promoted by crude cellular extracts, provide unequivocal confirmation that this mechanism does indeed operate in M. thermautotrophicus H.",

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author = "Lars Schomacher and Chong, {James P. J.} and Paul McDermott and Wilfried Kramer and Hans-Joachim Fritz",

year = "2009",

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T1 - DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision

AU - Schomacher, Lars

AU - Chong, James P. J.

AU - McDermott, Paul

AU - Kramer, Wilfried

AU - Fritz, Hans-Joachim

PY - 2009/4

Y1 - 2009/4

N2 - No genes for any of the known uracil DNA glycosylases of the UDG superfamily are present in the genome of Methanothermobacter thermautotrophicus H, making it difficult to imagine how DNA-U repair might be initiated in this organism. Recently, Mth212, the ExoIII homologue of M. thermautotrophicus H has been characterized as a DNA uridine endonuclease, which suggested the possibility of a novel endonucleolytic entry mechanism for DNA uracil repair. With no system of genetic experimentation available, the problem was approached biochemically. Assays of DNA uracil repair in vitro, promoted by crude cellular extracts, provide unequivocal confirmation that this mechanism does indeed operate in M. thermautotrophicus H.

AB - No genes for any of the known uracil DNA glycosylases of the UDG superfamily are present in the genome of Methanothermobacter thermautotrophicus H, making it difficult to imagine how DNA-U repair might be initiated in this organism. Recently, Mth212, the ExoIII homologue of M. thermautotrophicus H has been characterized as a DNA uridine endonuclease, which suggested the possibility of a novel endonucleolytic entry mechanism for DNA uracil repair. With no system of genetic experimentation available, the problem was approached biochemically. Assays of DNA uracil repair in vitro, promoted by crude cellular extracts, provide unequivocal confirmation that this mechanism does indeed operate in M. thermautotrophicus H.

KW - BASE EXCISION-REPAIR

KW - ESCHERICHIA-COLI K-12

KW - THERMOAUTOTROPHICUM DELTA-H

KW - COMPLETE GENOME SEQUENCE

KW - METHANOBACTERIUM-THERMOAUTOTROPHICUM

KW - METHANOTHERMOBACTER-THERMAUTOTROPHICUS

KW - MISMATCH ENDONUCLEASE

KW - EXONUCLEASE-III

KW - POLYMERASE

KW - GENE

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DO - 10.1093/nar/gkp102

M3 - Article

SN - 0305-1048

VL - 37

SP - 2283

EP - 2293

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 7

ER -

DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision

Abstract

Keywords

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