Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes

Sybrin P. Schröder; Casper De Boer; Nicholas G.S. McGregor; Rhianna J. Rowland; Olga Moroz; Elena Blagova; Jos Reijngoud; Mark Arentshorst; David Osborn; Marc D. Morant; Eric Abbate; Mary A. Stringer; Kristian B.R.M. Krogh; Lluís Raich; Carme Rovira; Jean Guy Berrin; Gilles P. Van Wezel; Arthur F.J. Ram; Bogdan I. Florea; Gijsbert A. Van Der Marel; Jeroen D.C. Codée; Keith S. Wilson; Liang Wu; Gideon J. Davies; Herman S. Overkleeft

doi:10.1021/acscentsci.9b00221

Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes

Sybrin P. Schröder, Casper De Boer, Nicholas G.S. McGregor, Rhianna J. Rowland, Olga Moroz, Elena Blagova, Jos Reijngoud, Mark Arentshorst, David Osborn, Marc D. Morant, Eric Abbate, Mary A. Stringer, Kristian B.R.M. Krogh, Lluís Raich, Carme Rovira, Jean Guy Berrin, Gilles P. Van Wezel, Arthur F.J. Ram, Bogdan I. Florea, Gijsbert A. Van Der MarelJeroen D.C. Codée, Keith S. Wilson, Liang Wu^*, Gideon J. Davies, Herman S. Overkleeft

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Plant polysaccharides represent a virtually unlimited feedstock for the generation of biofuels and other commodities. However, the extraordinary recalcitrance of plant polysaccharides toward breakdown necessitates a continued search for enzymes that degrade these materials efficiently under defined conditions. Activity-based protein profiling provides a route for the functional discovery of such enzymes in complex mixtures and under industrially relevant conditions. Here, we show the detection and identification of β-xylosidases and endo-β-1,4-xylanases in the secretomes of Aspergillus niger, by the use of chemical probes inspired by the β-glucosidase inhibitor cyclophellitol. Furthermore, we demonstrate the use of these activity-based probes (ABPs) to assess enzyme-substrate specificities, thermal stabilities, and other biotechnologically relevant parameters. Our experiments highlight the utility of ABPs as promising tools for the discovery of relevant enzymes useful for biomass breakdown.

Original language	English
Pages (from-to)	1067-1078
Number of pages	12
Journal	ACS Central Science
Volume	5
Issue number	6
Early online date	24 May 2019
DOIs	https://doi.org/10.1021/acscentsci.9b00221
Publication status	Published - 26 Jun 2019

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10.1021/acscentsci.9b00221Licence: Other

acscentsci.9b00221
© 2019 American Chemical Society.
Final published version, 8.92 MBLicence: CC BY

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Schröder, S. P., De Boer, C., McGregor, N. G. S., Rowland, R. J., Moroz, O., Blagova, E., Reijngoud, J., Arentshorst, M., Osborn, D., Morant, M. D., Abbate, E., Stringer, M. A., Krogh, K. B. R. M., Raich, L., Rovira, C., Berrin, J. G., Van Wezel, G. P., Ram, A. F. J., Florea, B. I., ... Overkleeft, H. S. (2019). Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes. ACS Central Science, 5(6), 1067-1078. https://doi.org/10.1021/acscentsci.9b00221

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title = "Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes",

abstract = "Plant polysaccharides represent a virtually unlimited feedstock for the generation of biofuels and other commodities. However, the extraordinary recalcitrance of plant polysaccharides toward breakdown necessitates a continued search for enzymes that degrade these materials efficiently under defined conditions. Activity-based protein profiling provides a route for the functional discovery of such enzymes in complex mixtures and under industrially relevant conditions. Here, we show the detection and identification of β-xylosidases and endo-β-1,4-xylanases in the secretomes of Aspergillus niger, by the use of chemical probes inspired by the β-glucosidase inhibitor cyclophellitol. Furthermore, we demonstrate the use of these activity-based probes (ABPs) to assess enzyme-substrate specificities, thermal stabilities, and other biotechnologically relevant parameters. Our experiments highlight the utility of ABPs as promising tools for the discovery of relevant enzymes useful for biomass breakdown.",

author = "Schr{\"o}der, {Sybrin P.} and {De Boer}, Casper and McGregor, {Nicholas G.S.} and Rowland, {Rhianna J.} and Olga Moroz and Elena Blagova and Jos Reijngoud and Mark Arentshorst and David Osborn and Morant, {Marc D.} and Eric Abbate and Stringer, {Mary A.} and Krogh, {Kristian B.R.M.} and Llu{\'i}s Raich and Carme Rovira and Berrin, {Jean Guy} and {Van Wezel}, {Gilles P.} and Ram, {Arthur F.J.} and Florea, {Bogdan I.} and {Van Der Marel}, {Gijsbert A.} and Cod{\'e}e, {Jeroen D.C.} and Wilson, {Keith S.} and Liang Wu and Davies, {Gideon J.} and Overkleeft, {Herman S.}",

note = " {\textcopyright} 2019 American Chemical Society.",

year = "2019",

month = jun,

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doi = "10.1021/acscentsci.9b00221",

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Schröder, SP, De Boer, C, McGregor, NGS, Rowland, RJ, Moroz, O , Blagova, E, Reijngoud, J, Arentshorst, M, Osborn, D, Morant, MD, Abbate, E, Stringer, MA, Krogh, KBRM, Raich, L, Rovira, C, Berrin, JG, Van Wezel, GP, Ram, AFJ, Florea, BI, Van Der Marel, GA, Codée, JDC, Wilson, KS , Wu, L , Davies, GJ & Overkleeft, HS 2019, 'Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes', ACS Central Science, vol. 5, no. 6, pp. 1067-1078. https://doi.org/10.1021/acscentsci.9b00221

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T1 - Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes

AU - Schröder, Sybrin P.

AU - De Boer, Casper

AU - McGregor, Nicholas G.S.

AU - Rowland, Rhianna J.

AU - Moroz, Olga

AU - Blagova, Elena

AU - Reijngoud, Jos

AU - Arentshorst, Mark

AU - Osborn, David

AU - Morant, Marc D.

AU - Abbate, Eric

AU - Stringer, Mary A.

AU - Krogh, Kristian B.R.M.

AU - Raich, Lluís

AU - Rovira, Carme

AU - Berrin, Jean Guy

AU - Van Wezel, Gilles P.

AU - Ram, Arthur F.J.

AU - Florea, Bogdan I.

AU - Van Der Marel, Gijsbert A.

AU - Codée, Jeroen D.C.

AU - Wilson, Keith S.

AU - Wu, Liang

AU - Davies, Gideon J.

AU - Overkleeft, Herman S.

PY - 2019/6/26

Y1 - 2019/6/26

N2 - Plant polysaccharides represent a virtually unlimited feedstock for the generation of biofuels and other commodities. However, the extraordinary recalcitrance of plant polysaccharides toward breakdown necessitates a continued search for enzymes that degrade these materials efficiently under defined conditions. Activity-based protein profiling provides a route for the functional discovery of such enzymes in complex mixtures and under industrially relevant conditions. Here, we show the detection and identification of β-xylosidases and endo-β-1,4-xylanases in the secretomes of Aspergillus niger, by the use of chemical probes inspired by the β-glucosidase inhibitor cyclophellitol. Furthermore, we demonstrate the use of these activity-based probes (ABPs) to assess enzyme-substrate specificities, thermal stabilities, and other biotechnologically relevant parameters. Our experiments highlight the utility of ABPs as promising tools for the discovery of relevant enzymes useful for biomass breakdown.

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U2 - 10.1021/acscentsci.9b00221

DO - 10.1021/acscentsci.9b00221

M3 - Article

AN - SCOPUS:85066455209

SN - 2374-7943

VL - 5

SP - 1067

EP - 1078

JO - ACS Central Science

JF - ACS Central Science

IS - 6

ER -

Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes

Abstract

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Keith Sanderson Wilson

MassIVE MSV000083714 - Activity-based protein profiling of xylan-degrading glycoside hydrolases in Aspergillus secretomes

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Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes

Abstract

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Keith Sanderson Wilson

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MassIVE MSV000083714 - Activity-based protein profiling of xylan-degrading glycoside hydrolases in Aspergillus secretomes

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