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Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes

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Author(s)

  • Sybrin P. Schröder
  • Casper De Boer
  • Rhianna J. Rowland
  • Jos Reijngoud
  • Mark Arentshorst
  • David Osborn
  • Marc D. Morant
  • Eric Abbate
  • Mary A. Stringer
  • Kristian B.R.M. Krogh
  • Lluís Raich
  • Carme Rovira
  • Jean Guy Berrin
  • Gilles P. Van Wezel
  • Arthur F.J. Ram
  • Bogdan I. Florea
  • Gijsbert A. Van Der Marel
  • Jeroen D.C. Codée
  • Herman S. Overkleeft

Department/unit(s)

Publication details

JournalACS Central Science
DateAccepted/In press - 14 May 2019
DateE-pub ahead of print - 24 May 2019
DatePublished (current) - 26 Jun 2019
Issue number6
Volume5
Number of pages12
Pages (from-to)1067-1078
Early online date24/05/19
Original languageEnglish

Abstract

Plant polysaccharides represent a virtually unlimited feedstock for the generation of biofuels and other commodities. However, the extraordinary recalcitrance of plant polysaccharides toward breakdown necessitates a continued search for enzymes that degrade these materials efficiently under defined conditions. Activity-based protein profiling provides a route for the functional discovery of such enzymes in complex mixtures and under industrially relevant conditions. Here, we show the detection and identification of β-xylosidases and endo-β-1,4-xylanases in the secretomes of Aspergillus niger, by the use of chemical probes inspired by the β-glucosidase inhibitor cyclophellitol. Furthermore, we demonstrate the use of these activity-based probes (ABPs) to assess enzyme-substrate specificities, thermal stabilities, and other biotechnologically relevant parameters. Our experiments highlight the utility of ABPs as promising tools for the discovery of relevant enzymes useful for biomass breakdown.

Bibliographical note

© 2019 American Chemical Society.

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