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Journal | ACS Central Science |
---|---|
Date | Accepted/In press - 14 May 2019 |
Date | E-pub ahead of print - 24 May 2019 |
Date | Published (current) - 26 Jun 2019 |
Issue number | 6 |
Volume | 5 |
Number of pages | 12 |
Pages (from-to) | 1067-1078 |
Early online date | 24/05/19 |
Original language | English |
Plant polysaccharides represent a virtually unlimited feedstock for the generation of biofuels and other commodities. However, the extraordinary recalcitrance of plant polysaccharides toward breakdown necessitates a continued search for enzymes that degrade these materials efficiently under defined conditions. Activity-based protein profiling provides a route for the functional discovery of such enzymes in complex mixtures and under industrially relevant conditions. Here, we show the detection and identification of β-xylosidases and endo-β-1,4-xylanases in the secretomes of Aspergillus niger, by the use of chemical probes inspired by the β-glucosidase inhibitor cyclophellitol. Furthermore, we demonstrate the use of these activity-based probes (ABPs) to assess enzyme-substrate specificities, thermal stabilities, and other biotechnologically relevant parameters. Our experiments highlight the utility of ABPs as promising tools for the discovery of relevant enzymes useful for biomass breakdown.
© 2019 American Chemical Society.
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