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Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes

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Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes. / Schröder, Sybrin P.; De Boer, Casper; McGregor, Nicholas G.S.; Rowland, Rhianna J.; Moroz, Olga; Blagova, Elena; Reijngoud, Jos; Arentshorst, Mark; Osborn, David; Morant, Marc D.; Abbate, Eric; Stringer, Mary A.; Krogh, Kristian B.R.M.; Raich, Lluís; Rovira, Carme; Berrin, Jean Guy; Van Wezel, Gilles P.; Ram, Arthur F.J.; Florea, Bogdan I.; Van Der Marel, Gijsbert A.; Codée, Jeroen D.C.; Wilson, Keith S.; Wu, Liang; Davies, Gideon J.; Overkleeft, Herman S.

In: ACS Central Science, Vol. 5, No. 6, 26.06.2019, p. 1067-1078.

Research output: Contribution to journalArticlepeer-review

Harvard

Schröder, SP, De Boer, C, McGregor, NGS, Rowland, RJ, Moroz, O, Blagova, E, Reijngoud, J, Arentshorst, M, Osborn, D, Morant, MD, Abbate, E, Stringer, MA, Krogh, KBRM, Raich, L, Rovira, C, Berrin, JG, Van Wezel, GP, Ram, AFJ, Florea, BI, Van Der Marel, GA, Codée, JDC, Wilson, KS, Wu, L, Davies, GJ & Overkleeft, HS 2019, 'Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes', ACS Central Science, vol. 5, no. 6, pp. 1067-1078. https://doi.org/10.1021/acscentsci.9b00221

APA

Schröder, S. P., De Boer, C., McGregor, N. G. S., Rowland, R. J., Moroz, O., Blagova, E., Reijngoud, J., Arentshorst, M., Osborn, D., Morant, M. D., Abbate, E., Stringer, M. A., Krogh, K. B. R. M., Raich, L., Rovira, C., Berrin, J. G., Van Wezel, G. P., Ram, A. F. J., Florea, B. I., ... Overkleeft, H. S. (2019). Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes. ACS Central Science, 5(6), 1067-1078. https://doi.org/10.1021/acscentsci.9b00221

Vancouver

Schröder SP, De Boer C, McGregor NGS, Rowland RJ, Moroz O, Blagova E et al. Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes. ACS Central Science. 2019 Jun 26;5(6):1067-1078. https://doi.org/10.1021/acscentsci.9b00221

Author

Schröder, Sybrin P. ; De Boer, Casper ; McGregor, Nicholas G.S. ; Rowland, Rhianna J. ; Moroz, Olga ; Blagova, Elena ; Reijngoud, Jos ; Arentshorst, Mark ; Osborn, David ; Morant, Marc D. ; Abbate, Eric ; Stringer, Mary A. ; Krogh, Kristian B.R.M. ; Raich, Lluís ; Rovira, Carme ; Berrin, Jean Guy ; Van Wezel, Gilles P. ; Ram, Arthur F.J. ; Florea, Bogdan I. ; Van Der Marel, Gijsbert A. ; Codée, Jeroen D.C. ; Wilson, Keith S. ; Wu, Liang ; Davies, Gideon J. ; Overkleeft, Herman S. / Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes. In: ACS Central Science. 2019 ; Vol. 5, No. 6. pp. 1067-1078.

Bibtex - Download

@article{4c590ad148e342b984d3427e93be6395,
title = "Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes",
abstract = "Plant polysaccharides represent a virtually unlimited feedstock for the generation of biofuels and other commodities. However, the extraordinary recalcitrance of plant polysaccharides toward breakdown necessitates a continued search for enzymes that degrade these materials efficiently under defined conditions. Activity-based protein profiling provides a route for the functional discovery of such enzymes in complex mixtures and under industrially relevant conditions. Here, we show the detection and identification of β-xylosidases and endo-β-1,4-xylanases in the secretomes of Aspergillus niger, by the use of chemical probes inspired by the β-glucosidase inhibitor cyclophellitol. Furthermore, we demonstrate the use of these activity-based probes (ABPs) to assess enzyme-substrate specificities, thermal stabilities, and other biotechnologically relevant parameters. Our experiments highlight the utility of ABPs as promising tools for the discovery of relevant enzymes useful for biomass breakdown.",
author = "Schr{\"o}der, {Sybrin P.} and {De Boer}, Casper and McGregor, {Nicholas G.S.} and Rowland, {Rhianna J.} and Olga Moroz and Elena Blagova and Jos Reijngoud and Mark Arentshorst and David Osborn and Morant, {Marc D.} and Eric Abbate and Stringer, {Mary A.} and Krogh, {Kristian B.R.M.} and Llu{\'i}s Raich and Carme Rovira and Berrin, {Jean Guy} and {Van Wezel}, {Gilles P.} and Ram, {Arthur F.J.} and Florea, {Bogdan I.} and {Van Der Marel}, {Gijsbert A.} and Cod{\'e}e, {Jeroen D.C.} and Wilson, {Keith S.} and Liang Wu and Davies, {Gideon J.} and Overkleeft, {Herman S.}",
note = " {\textcopyright} 2019 American Chemical Society.",
year = "2019",
month = jun,
day = "26",
doi = "10.1021/acscentsci.9b00221",
language = "English",
volume = "5",
pages = "1067--1078",
journal = "ACS Central Science",
issn = "2374-7943",
publisher = "American Chemical Society",
number = "6",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes

AU - Schröder, Sybrin P.

AU - De Boer, Casper

AU - McGregor, Nicholas G.S.

AU - Rowland, Rhianna J.

AU - Moroz, Olga

AU - Blagova, Elena

AU - Reijngoud, Jos

AU - Arentshorst, Mark

AU - Osborn, David

AU - Morant, Marc D.

AU - Abbate, Eric

AU - Stringer, Mary A.

AU - Krogh, Kristian B.R.M.

AU - Raich, Lluís

AU - Rovira, Carme

AU - Berrin, Jean Guy

AU - Van Wezel, Gilles P.

AU - Ram, Arthur F.J.

AU - Florea, Bogdan I.

AU - Van Der Marel, Gijsbert A.

AU - Codée, Jeroen D.C.

AU - Wilson, Keith S.

AU - Wu, Liang

AU - Davies, Gideon J.

AU - Overkleeft, Herman S.

N1 - © 2019 American Chemical Society.

PY - 2019/6/26

Y1 - 2019/6/26

N2 - Plant polysaccharides represent a virtually unlimited feedstock for the generation of biofuels and other commodities. However, the extraordinary recalcitrance of plant polysaccharides toward breakdown necessitates a continued search for enzymes that degrade these materials efficiently under defined conditions. Activity-based protein profiling provides a route for the functional discovery of such enzymes in complex mixtures and under industrially relevant conditions. Here, we show the detection and identification of β-xylosidases and endo-β-1,4-xylanases in the secretomes of Aspergillus niger, by the use of chemical probes inspired by the β-glucosidase inhibitor cyclophellitol. Furthermore, we demonstrate the use of these activity-based probes (ABPs) to assess enzyme-substrate specificities, thermal stabilities, and other biotechnologically relevant parameters. Our experiments highlight the utility of ABPs as promising tools for the discovery of relevant enzymes useful for biomass breakdown.

AB - Plant polysaccharides represent a virtually unlimited feedstock for the generation of biofuels and other commodities. However, the extraordinary recalcitrance of plant polysaccharides toward breakdown necessitates a continued search for enzymes that degrade these materials efficiently under defined conditions. Activity-based protein profiling provides a route for the functional discovery of such enzymes in complex mixtures and under industrially relevant conditions. Here, we show the detection and identification of β-xylosidases and endo-β-1,4-xylanases in the secretomes of Aspergillus niger, by the use of chemical probes inspired by the β-glucosidase inhibitor cyclophellitol. Furthermore, we demonstrate the use of these activity-based probes (ABPs) to assess enzyme-substrate specificities, thermal stabilities, and other biotechnologically relevant parameters. Our experiments highlight the utility of ABPs as promising tools for the discovery of relevant enzymes useful for biomass breakdown.

UR - http://www.scopus.com/inward/record.url?scp=85066455209&partnerID=8YFLogxK

U2 - 10.1021/acscentsci.9b00221

DO - 10.1021/acscentsci.9b00221

M3 - Article

AN - SCOPUS:85066455209

VL - 5

SP - 1067

EP - 1078

JO - ACS Central Science

JF - ACS Central Science

SN - 2374-7943

IS - 6

ER -