Early transcriptional targets of MyoD link myogenesis and somitogenesis

Richard J Maguire; Harry V Isaacs; Mary Elizabeth Pownall

doi:10.1016/j.ydbio.2012.08.027

Early transcriptional targets of MyoD link myogenesis and somitogenesis

Richard J Maguire, Harry V Isaacs, Mary Elizabeth Pownall

Biology

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Abstract

In order to identify early transcriptional targets of MyoD prior to skeletal muscle differentiation, we have undertaken a transcriptomic analysis on gastrula stage Xenopus embryos in which MyoD has been knocked-down. Our validated list of genes transcriptionally regulated by MyoD includes Esr1 and Esr2, which are known targets of Notch signalling, and Tbx6, mesogenin, and FoxC1; these genes are all are known to be essential for normal somitogenesis but are expressed surprisingly early in the mesoderm. In addition we found that MyoD is required for the expression of myf5 in the early mesoderm, in contrast to the reverse relationship of these two regulators in amniote somites. These data highlight a role for MyoD in the early mesoderm in regulating a set of genes that are essential for both myogenesis and somitogenesis.

Original language	English
Pages (from-to)	256-268
Number of pages	13
Journal	Developmental Biology
Volume	371
Issue number	2
DOIs	https://doi.org/10.1016/j.ydbio.2012.08.027
Publication status	Published - 15 Nov 2012

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10.1016/j.ydbio.2012.08.027

http://www.sciencedirect.com/science/article/pii/S001216061200471X

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title = "Early transcriptional targets of MyoD link myogenesis and somitogenesis",

abstract = "In order to identify early transcriptional targets of MyoD prior to skeletal muscle differentiation, we have undertaken a transcriptomic analysis on gastrula stage Xenopus embryos in which MyoD has been knocked-down. Our validated list of genes transcriptionally regulated by MyoD includes Esr1 and Esr2, which are known targets of Notch signalling, and Tbx6, mesogenin, and FoxC1; these genes are all are known to be essential for normal somitogenesis but are expressed surprisingly early in the mesoderm. In addition we found that MyoD is required for the expression of myf5 in the early mesoderm, in contrast to the reverse relationship of these two regulators in amniote somites. These data highlight a role for MyoD in the early mesoderm in regulating a set of genes that are essential for both myogenesis and somitogenesis.",

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Early transcriptional targets of MyoD link myogenesis and somitogenesis

Abstract

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