Effect of buffer at nanoscale molecular recognition interfaces - electrostatic binding of biological polyanions

Ana Campo Rodrigo; Erik Laurini; Vania Margarida Pinto Vieira; Sabrina Pricl; David Kelham Smith

Effect of buffer at nanoscale molecular recognition interfaces - electrostatic binding of biological polyanions

Ana Campo Rodrigo, Erik Laurini, Vania Margarida Pinto Vieira, Sabrina Pricl, David Kelham Smith

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

We investigate the impact of an over-looked component on molecular recognition in water–buffer. The binding of a cationic dye to biological polyanion heparin is shown by isothermal calorimetry to depend on buffer (Tris–HCl > HEPES > PBS). The heparin binding of self-assembled multivalent (SAMul) cationic micelles is even more buffer dependent. Multivalent electrostatic molecular recognition is buffer dependent as a result of competitive interactions between the cationic binding interface and anions present in the buffer.

Original language	English
Pages (from-to)	11580-11583
Journal	Chemical communications
Volume	53
Publication status	Published - 9 Oct 2017

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Ana Buffer Chem Comm 2017
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Cite this

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title = "Effect of buffer at nanoscale molecular recognition interfaces - electrostatic binding of biological polyanions",

abstract = "We investigate the impact of an over-looked component on molecular recognition in water–buffer. The binding of a cationic dye to biological polyanion heparin is shown by isothermal calorimetry to depend on buffer (Tris–HCl > HEPES > PBS). The heparin binding of self-assembled multivalent (SAMul) cationic micelles is even more buffer dependent. Multivalent electrostatic molecular recognition is buffer dependent as a result of competitive interactions between the cationic binding interface and anions present in the buffer.",

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AU - Campo Rodrigo, Ana

AU - Laurini, Erik

AU - Vieira, Vania Margarida Pinto

AU - Pricl, Sabrina

AU - Smith, David Kelham

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AB - We investigate the impact of an over-looked component on molecular recognition in water–buffer. The binding of a cationic dye to biological polyanion heparin is shown by isothermal calorimetry to depend on buffer (Tris–HCl > HEPES > PBS). The heparin binding of self-assembled multivalent (SAMul) cationic micelles is even more buffer dependent. Multivalent electrostatic molecular recognition is buffer dependent as a result of competitive interactions between the cationic binding interface and anions present in the buffer.

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JO - Chemical communications

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