Effect of the beta-1 subunit on electrophysiological properties of voltage-gated Na+ channels in MDA-MB-231 breast cancer cells

TY - CONF

T1 - Effect of the beta-1 subunit on electrophysiological properties of voltage-gated Na+ channels in MDA-MB-231 breast cancer cells

AU - Patel, Faheemmuddeen

AU - Brackenbury, William John

PY - 2017

Y1 - 2017

N2 - Introduction: Voltage-gated Na+ channels (VGSCs) are heteromeric protein complexes formed of a pore-forming α-subunit and one or more auxiliary β subunits. VGSCs increase invasion and migration in the triple-negative MDA-MB-231 breast cancer cell line. Overexpressing β1 in MDA-MB-231 cells (MDA-MB-231-β1-GFP) increases peak Na+ current density, although the mechanism(s) are unclear(1). β subunits are proteolytically processed by secretases, releasing intracellular domains (ICDs), which may in turn transcriptionally upregulate α-subunits(2). In this study, we aimed to investigate the mechanism by which β1 increases Na+ current in MDA-MB-231 cells.Method: Whole-cell patch clamp recording was used to isolate and quantify VGSC currents in voltage clamp mode. Cells were perfused with either 1 μM TTX or PF-04856042, an NaV1.7-specific blocker. To inhibit γ secretase, cells were treated with DAPT (0.5 µM), for 24 hours prior to recording.Results: Application of 1 µM TTX significantly reduced the peak Na+ current density in MDA-MB-231-β1-GFP cells from -14.3 ± 2.6 pA/pF to -9.6 ± 1.8 pA/pF (n=12, P<0.01) whilst having no significant effect in control MDA-MB-231-GFP cells. Treatment of MDA-MB-231-β1-GFP cells with DAPT significantly reduced the peak Na+ current density from -19.5 ± 2.4 pA/pF to -4.3 ± 2.7 pA/pF (n=12, P<0.001) but did not significantly alter the current density in control MDA-MB-231-GFP cells. Perfusing MDA-MB-231-β1-GFP cells with PF-04856042 significantly reduced the peak Na+ current density from -19.7 ± 2.6 pA/pF to -5.0 ± 0.6 pA/pF (n=10, P<0.001) but had no significant effect in control MDA-MB-231-GFP cells. NaV1.7, which is up-regulated in lung cancer cells, displays a unique subthreshold current(3, 4). A depolarising ramp protocol revealed an inward current in MDA-MB-231-β1-GFP, but not MDA-MB-231-GFP cells, consistent with Nav1.7 expression in the former. Perfusion with PF-04856042 significantly inhibited this current in MDA-MB-231-β1-GFP cells, but had no effect in control MDA-MB-231-GFP cells (n=10, P<0.0001).Conclusion: Our data suggest that β1 up-regulates the expression of NaV1.7 in MDA-MB-231 cells, likely via a mechanism that requires proteolytic release of the β1-ICD. Combining PF-04856042 application with ramp recording may represent a useful new approach for studying NaV1.7 expression/activity in cancer cells.(1) CHIONI AM. et al. (2009). Int J Biochem Cell Biol 41: 1216-1227.References:(2) KIM DY et al. (2007). Nat Cell Biol 9: 755-64. (3) CUMMINS TR et al. (2009). Nat Protoc 4: 1103-12.CAMPBELL TM. et al. (2013). J Cell Sci 126: 4939-4949.

AB - Introduction: Voltage-gated Na+ channels (VGSCs) are heteromeric protein complexes formed of a pore-forming α-subunit and one or more auxiliary β subunits. VGSCs increase invasion and migration in the triple-negative MDA-MB-231 breast cancer cell line. Overexpressing β1 in MDA-MB-231 cells (MDA-MB-231-β1-GFP) increases peak Na+ current density, although the mechanism(s) are unclear(1). β subunits are proteolytically processed by secretases, releasing intracellular domains (ICDs), which may in turn transcriptionally upregulate α-subunits(2). In this study, we aimed to investigate the mechanism by which β1 increases Na+ current in MDA-MB-231 cells.Method: Whole-cell patch clamp recording was used to isolate and quantify VGSC currents in voltage clamp mode. Cells were perfused with either 1 μM TTX or PF-04856042, an NaV1.7-specific blocker. To inhibit γ secretase, cells were treated with DAPT (0.5 µM), for 24 hours prior to recording.Results: Application of 1 µM TTX significantly reduced the peak Na+ current density in MDA-MB-231-β1-GFP cells from -14.3 ± 2.6 pA/pF to -9.6 ± 1.8 pA/pF (n=12, P<0.01) whilst having no significant effect in control MDA-MB-231-GFP cells. Treatment of MDA-MB-231-β1-GFP cells with DAPT significantly reduced the peak Na+ current density from -19.5 ± 2.4 pA/pF to -4.3 ± 2.7 pA/pF (n=12, P<0.001) but did not significantly alter the current density in control MDA-MB-231-GFP cells. Perfusing MDA-MB-231-β1-GFP cells with PF-04856042 significantly reduced the peak Na+ current density from -19.7 ± 2.6 pA/pF to -5.0 ± 0.6 pA/pF (n=10, P<0.001) but had no significant effect in control MDA-MB-231-GFP cells. NaV1.7, which is up-regulated in lung cancer cells, displays a unique subthreshold current(3, 4). A depolarising ramp protocol revealed an inward current in MDA-MB-231-β1-GFP, but not MDA-MB-231-GFP cells, consistent with Nav1.7 expression in the former. Perfusion with PF-04856042 significantly inhibited this current in MDA-MB-231-β1-GFP cells, but had no effect in control MDA-MB-231-GFP cells (n=10, P<0.0001).Conclusion: Our data suggest that β1 up-regulates the expression of NaV1.7 in MDA-MB-231 cells, likely via a mechanism that requires proteolytic release of the β1-ICD. Combining PF-04856042 application with ramp recording may represent a useful new approach for studying NaV1.7 expression/activity in cancer cells.(1) CHIONI AM. et al. (2009). Int J Biochem Cell Biol 41: 1216-1227.References:(2) KIM DY et al. (2007). Nat Cell Biol 9: 755-64. (3) CUMMINS TR et al. (2009). Nat Protoc 4: 1103-12.CAMPBELL TM. et al. (2013). J Cell Sci 126: 4939-4949.

M3 - Abstract

T2 - Pharmacology 2016

Y2 - 13 December 2016 through 15 December 2016

ER -