By the same authors

From the same journal

Effects of microcompartmentation on flux distribution and metabolic pools in Chlamydomonas reinhardtii chloroplasts

Research output: Contribution to journalArticle

Author(s)

  • A. Kuken
  • F. Sommer
  • L. Yaneva-Roder
  • Luke Mackinder
  • M. Hohne
  • S. Geimer
  • Martin C. Jonikas
  • M. Schroda
  • M. Stitt
  • Z. Nikoloski
  • Tabea Mettler-Altmann

Department/unit(s)

Publication details

JournaleLife
DateAccepted/In press - 28 Sep 2018
DatePublished (current) - 11 Oct 2018
Number of pages23
Original languageEnglish

Abstract

Cells and organelles are not homogeneous but include microcompartments that alter the spatiotemporal characteristics of cellular processes. The effects of microcompartmentation on metabolic pathways are however difficult to study experimentally. The pyrenoid is a microcompartment that is essential for a carbon concentrating mechanism (CCM) that improves the photosynthetic performance of eukaryotic algae. Using Chlamydomonas reinhardtii, we obtained experimental data on photosynthesis, metabolites, and proteins in CCM-induced and CCM-suppressed cells. We then employed a computational strategy to estimate how fluxes through the Calvin-Benson cycle are compartmented between the pyrenoid and the stroma. Our model predicts that ribulose-1,5-bisphosphate (RuBP), the substrate of Rubisco, and 3-phosphoglycerate (3PGA), its product, diffuse in and out of the pyrenoid, respectively, with higher fluxes in CCM-induced cells. It also indicates that there is no major diffusional barrier to metabolic flux between the pyrenoid and stroma. Our computational approach represents a stepping stone to understanding microcompartmentalized CCM in other organisms.

Bibliographical note

©Kuken et al.

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