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Efficient N-terminal labeling of proteins by use of sortase

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JournalAngewandte Chemie International Edition
DatePublished - 10 Sep 2012
Issue number37
Volume51
Pages (from-to)9377-9380
Original languageEnglish

Abstract

"Sorting out" N-terminal labeling: The reversibility of transpeptidase reactions makes protein N-terminal labeling challenging. Depsipeptide substrates for sortase A release alcohol by-products, which are poor nucleophiles for the reverse reaction, during ligation. Proteins with an unhindered N-terminal glycine residue can be labeled efficiently with only a minimal excess of the labeling reagent (see scheme).

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