Electrophoretic method for assessment of substrate promiscuity of a heterogeneous biocatalyst using an area imaging ultraviolet detector

Pawel L. Urban; Edmund T. Bergstroem; David M. Goodall; Sreedevi Narayanaswamy; Neil C. Bruce

doi:10.1039/b710495b

Electrophoretic method for assessment of substrate promiscuity of a heterogeneous biocatalyst using an area imaging ultraviolet detector

Pawel L. Urban, Edmund T. Bergstroem, David M. Goodall, Sreedevi Narayanaswamy, Neil C. Bruce

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Abstract

We report a new electrophoretic set-up and method for rapid specificity screening of an immobilised enzyme against a range of substrates present in a mixture. The penicillinase-catalysed reaction is carried out on-the-fly, following separation of putative substrates and preceding separation of the reaction products. The new active pixel sensor detector gives an option of using multiple detection windows on a single flow line and enables efficient on-line monitoring of this heterogeneous biocatalytic process with multiple putative substrates injected simultaneously.

Original language	English
Pages (from-to)	979-982
Number of pages	4
Journal	Analyst
Volume	132
Issue number	10
DOIs	https://doi.org/10.1039/b710495b
Publication status	Published - 2007

Keywords

CAPILLARY-ELECTROPHORESIS
ENZYME PROMISCUITY
MASS-SPECTROMETRY
ASSAY
CE

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10.1039/b710495b

Cite this

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title = "Electrophoretic method for assessment of substrate promiscuity of a heterogeneous biocatalyst using an area imaging ultraviolet detector",

abstract = "We report a new electrophoretic set-up and method for rapid specificity screening of an immobilised enzyme against a range of substrates present in a mixture. The penicillinase-catalysed reaction is carried out on-the-fly, following separation of putative substrates and preceding separation of the reaction products. The new active pixel sensor detector gives an option of using multiple detection windows on a single flow line and enables efficient on-line monitoring of this heterogeneous biocatalytic process with multiple putative substrates injected simultaneously.",

keywords = "CAPILLARY-ELECTROPHORESIS, ENZYME PROMISCUITY, MASS-SPECTROMETRY, ASSAY, CE",

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T1 - Electrophoretic method for assessment of substrate promiscuity of a heterogeneous biocatalyst using an area imaging ultraviolet detector

AU - Urban, Pawel L.

AU - Bergstroem, Edmund T.

AU - Goodall, David M.

AU - Narayanaswamy, Sreedevi

AU - Bruce, Neil C.

PY - 2007

Y1 - 2007

N2 - We report a new electrophoretic set-up and method for rapid specificity screening of an immobilised enzyme against a range of substrates present in a mixture. The penicillinase-catalysed reaction is carried out on-the-fly, following separation of putative substrates and preceding separation of the reaction products. The new active pixel sensor detector gives an option of using multiple detection windows on a single flow line and enables efficient on-line monitoring of this heterogeneous biocatalytic process with multiple putative substrates injected simultaneously.

AB - We report a new electrophoretic set-up and method for rapid specificity screening of an immobilised enzyme against a range of substrates present in a mixture. The penicillinase-catalysed reaction is carried out on-the-fly, following separation of putative substrates and preceding separation of the reaction products. The new active pixel sensor detector gives an option of using multiple detection windows on a single flow line and enables efficient on-line monitoring of this heterogeneous biocatalytic process with multiple putative substrates injected simultaneously.

KW - CAPILLARY-ELECTROPHORESIS

KW - ENZYME PROMISCUITY

KW - MASS-SPECTROMETRY

KW - ASSAY

KW - CE

U2 - 10.1039/b710495b

DO - 10.1039/b710495b

M3 - Article

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EP - 982

JO - Analyst

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