By the same authors

From the same journal

Electrophoretic method for assessment of substrate promiscuity of a heterogeneous biocatalyst using an area imaging ultraviolet detector

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Published copy (DOI)

Author(s)

  • Pawel L. Urban
  • Edmund T. Bergstroem
  • David M. Goodall
  • Sreedevi Narayanaswamy
  • Neil C. Bruce

Department/unit(s)

Publication details

JournalAnalyst
DatePublished - 2007
Issue number10
Volume132
Number of pages4
Pages (from-to)979-982
Original languageEnglish

Abstract

We report a new electrophoretic set-up and method for rapid specificity screening of an immobilised enzyme against a range of substrates present in a mixture. The penicillinase-catalysed reaction is carried out on-the-fly, following separation of putative substrates and preceding separation of the reaction products. The new active pixel sensor detector gives an option of using multiple detection windows on a single flow line and enables efficient on-line monitoring of this heterogeneous biocatalytic process with multiple putative substrates injected simultaneously.

    Research areas

  • CAPILLARY-ELECTROPHORESIS, ENZYME PROMISCUITY, MASS-SPECTROMETRY, ASSAY, CE

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