Electrophoretically mediated microanalysis of a nicotinamide adenine dinucleotide-dependent enzyme and its facile multiplexing using an active pixel sensor UV detector

Pawel L. Urban; David M. Goodall; Edmund T. Bergstroem; Neil C. Bruce

doi:10.1016/j.chroma.2007.04.071

Electrophoretically mediated microanalysis of a nicotinamide adenine dinucleotide-dependent enzyme and its facile multiplexing using an active pixel sensor UV detector

Pawel L. Urban, David M. Goodall, Edmund T. Bergstroem, Neil C. Bruce

CNAP

Research output: Contribution to journal › Article › peer-review

Abstract

An electrophoretically mediated microanalysis (EMMA) method has been developed for yeast alcohol dehydrogenase and quantification of reactant and product cofactors, NAD and NADH. The enzyme substrate ethanol (1% (v/v)) was added to the buffer (50 mM borate, pH 8.8). Results are presented for parallel capillary electrophoresis with a novel miniature UV area detector, with an active pixel sensor imaging an array of two or six parallel capillaries connected via a manifold to a single output capillary in a commercial CE instrument, allowing conversions with five different yeast alcohol dehydrogenase concentrations to be quantified in a single experiment. (c) 2007 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	132-140
Number of pages	9
Journal	Journal of Chromatography A
Volume	1162
Issue number	2
DOIs	https://doi.org/10.1016/j.chroma.2007.04.071
Publication status	Published - 31 Aug 2007

Keywords

active pixel sensor
capillary electrophoresis
EMMA
enzyme assay
NAD
plug-plug
CAPILLARY-ZONE-ELECTROPHORESIS
GLUCOSE-OXIDASE
ASSAY
EVOLUTION
COMBINATORIAL
WAVELENGTH
ABSORBENCY
CE

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10.1016/j.chroma.2007.04.071

http://www.sciencedirect.com/science/article/pii/S0021967307007509

Cite this

@article{5c72ca8723854dd3a2d4ca72ef1b2b00,

title = "Electrophoretically mediated microanalysis of a nicotinamide adenine dinucleotide-dependent enzyme and its facile multiplexing using an active pixel sensor UV detector",

abstract = "An electrophoretically mediated microanalysis (EMMA) method has been developed for yeast alcohol dehydrogenase and quantification of reactant and product cofactors, NAD and NADH. The enzyme substrate ethanol (1% (v/v)) was added to the buffer (50 mM borate, pH 8.8). Results are presented for parallel capillary electrophoresis with a novel miniature UV area detector, with an active pixel sensor imaging an array of two or six parallel capillaries connected via a manifold to a single output capillary in a commercial CE instrument, allowing conversions with five different yeast alcohol dehydrogenase concentrations to be quantified in a single experiment. (c) 2007 Elsevier B.V. All rights reserved.",

keywords = "active pixel sensor, capillary electrophoresis, EMMA, enzyme assay, NAD, plug-plug, CAPILLARY-ZONE-ELECTROPHORESIS, GLUCOSE-OXIDASE, ASSAY, EVOLUTION, COMBINATORIAL, WAVELENGTH, ABSORBENCY, CE",

author = "Urban, {Pawel L.} and Goodall, {David M.} and Bergstroem, {Edmund T.} and Bruce, {Neil C.}",

year = "2007",

month = aug,

day = "31",

doi = "10.1016/j.chroma.2007.04.071",

language = "English",

volume = "1162",

pages = "132--140",

journal = "Journal of Chromatography A",

issn = "0021-9673",

publisher = "Elsevier",

number = "2",

}

Electrophoretically mediated microanalysis of a nicotinamide adenine dinucleotide-dependent enzyme and its facile multiplexing using an active pixel sensor UV detector. / Urban, Pawel L.; Goodall, David M.; Bergstroem, Edmund T. et al.
In: Journal of Chromatography A, Vol. 1162, No. 2, 31.08.2007, p. 132-140.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Electrophoretically mediated microanalysis of a nicotinamide adenine dinucleotide-dependent enzyme and its facile multiplexing using an active pixel sensor UV detector

AU - Urban, Pawel L.

AU - Goodall, David M.

AU - Bergstroem, Edmund T.

AU - Bruce, Neil C.

PY - 2007/8/31

Y1 - 2007/8/31

N2 - An electrophoretically mediated microanalysis (EMMA) method has been developed for yeast alcohol dehydrogenase and quantification of reactant and product cofactors, NAD and NADH. The enzyme substrate ethanol (1% (v/v)) was added to the buffer (50 mM borate, pH 8.8). Results are presented for parallel capillary electrophoresis with a novel miniature UV area detector, with an active pixel sensor imaging an array of two or six parallel capillaries connected via a manifold to a single output capillary in a commercial CE instrument, allowing conversions with five different yeast alcohol dehydrogenase concentrations to be quantified in a single experiment. (c) 2007 Elsevier B.V. All rights reserved.

AB - An electrophoretically mediated microanalysis (EMMA) method has been developed for yeast alcohol dehydrogenase and quantification of reactant and product cofactors, NAD and NADH. The enzyme substrate ethanol (1% (v/v)) was added to the buffer (50 mM borate, pH 8.8). Results are presented for parallel capillary electrophoresis with a novel miniature UV area detector, with an active pixel sensor imaging an array of two or six parallel capillaries connected via a manifold to a single output capillary in a commercial CE instrument, allowing conversions with five different yeast alcohol dehydrogenase concentrations to be quantified in a single experiment. (c) 2007 Elsevier B.V. All rights reserved.

KW - active pixel sensor

KW - capillary electrophoresis

KW - EMMA

KW - enzyme assay

KW - NAD

KW - plug-plug

KW - CAPILLARY-ZONE-ELECTROPHORESIS

KW - GLUCOSE-OXIDASE

KW - ASSAY

KW - EVOLUTION

KW - COMBINATORIAL

KW - WAVELENGTH

KW - ABSORBENCY

KW - CE

U2 - 10.1016/j.chroma.2007.04.071

DO - 10.1016/j.chroma.2007.04.071

M3 - Article

SN - 0021-9673

VL - 1162

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JO - Journal of Chromatography A

JF - Journal of Chromatography A

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ER -