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Electrophoretically mediated microanalysis of a nicotinamide adenine dinucleotide-dependent enzyme and its facile multiplexing using an active pixel sensor UV detector

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JournalJournal of Chromatography A
DatePublished - 31 Aug 2007
Issue number2
Volume1162
Number of pages9
Pages (from-to)132-140
Original languageEnglish

Abstract

An electrophoretically mediated microanalysis (EMMA) method has been developed for yeast alcohol dehydrogenase and quantification of reactant and product cofactors, NAD and NADH. The enzyme substrate ethanol (1% (v/v)) was added to the buffer (50 mM borate, pH 8.8). Results are presented for parallel capillary electrophoresis with a novel miniature UV area detector, with an active pixel sensor imaging an array of two or six parallel capillaries connected via a manifold to a single output capillary in a commercial CE instrument, allowing conversions with five different yeast alcohol dehydrogenase concentrations to be quantified in a single experiment. (c) 2007 Elsevier B.V. All rights reserved.

    Research areas

  • active pixel sensor, capillary electrophoresis, EMMA, enzyme assay, NAD, plug-plug, CAPILLARY-ZONE-ELECTROPHORESIS, GLUCOSE-OXIDASE, ASSAY, EVOLUTION, COMBINATORIAL, WAVELENGTH, ABSORBENCY, CE

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