Burkholderia symbiotica sp. nov., isolated from root nodules of Mimosa spp. native to north-east Brazil

Shih-Yi Sheu, Jui-Hsing Chou, Cyril Bontemps, Geoffrey N. Elliott, Eduardo Gross, Euan K. James, Janet I. Sprent, Peter Young, Wen-Ming Chen

Research output: Contribution to journalArticlepeer-review


Four strains, JPY345T, JPY347, JPY366 and JPY581 were isolated from nitrogen-fixing nodules on the roots of two Mimosa spp. (M. cordistipula, M. misera) native to North East Brazil, and their taxonomic positions were investigated by a polyphasic approach. All four strains grew at 15-43 °C (optimum, 35 &degC), at pH 4.0-7.0 (optimum, pH 5.0) and with 0-2 % NaCl (optimum, 0 %). On the basis of 16S rRNA gene sequence analysis, a representative strain (JPY345T) showed 97.3 % sequence similarity to the closest species Burkholderia soli GP25-8T, a similarity of 97.3 % to Burkholderia caryophylli ATCC25418T and of 97.1 % to Burkholderia kururiensis KP23T. The predominant fatty acids of the strains were C18:1 ω7c (36.1 %), C16:0 (19.8 %) and summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c; 11.5 %). The major isoprenoid quinone was Q-8 and the DNA G+C content of the strains was 64.2-65.7 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized aminophospholipids and phospholipids. The DNA-DNA relatedness of the novel strain with respect to recognized species of the genus Burkholderia was less than 70 %. On the basis of 16S rRNA and recA gene sequence similarities, chemotaxonomic and phenotypic data, all the strains represent a novel species in the genus Burkholderia, for which the name Burkholderia symbiotica sp. nov. with the type strain JPY345T is proposed (=LMG 26032T=BCRC 80258T=KCTC 23309T).
Original languageEnglish
Pages (from-to)2272-2278
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Issue number9
Publication statusPublished - Sep 2012

Cite this