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Emergent mechanistic diversity of enzyme-catalysed beta-diketone cleavage

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JournalBiochemical journal
DatePublished - 15 Jun 2005
Volume388
Number of pages10
Pages (from-to)721-730
Original languageEnglish

Abstract

The enzymatic cleavage of C-C bonds in beta-diketones is, comparatively, a little studied biochemical process, but one that has important relevance to human metabolism, bioremediation and preparative biocatalysis. In recent studies, four types of enzymes have come to light that cleave C-C bonds in the beta-diketone functionality using different chemical mechanisms. OPH [oxidized poly(vinyl alcohol) hydrolase from Pseudomonas sp. strain VM15C], which cleaves nonane-4,6-dione to butyrate and pentan-2-one is a serine-triad hydrolase. Dke1 (diketone-cleaving enzyme from Acinetobacter johnsonii) is a dioxygenase, cleaving acetylacetone to methylglyoxal and acetate. Fumarylacetoacetate hydrolase cleaves fumarylacetoacetate to fumarate and acetoacetate using a water molecule, activated by a catalytic His/Asp dyad, aided by a calcium ion that both chelates the enol acid form of the substrate and indirectly positions the water for nucleophilic attack at a carbonyl group. 6-Oxocamphor hydrolase cleaves nonenolizable cyclic beta-diketones and is a homologue of the crotonase superfamily, employing a catalytic His/Asp dyad to activate a water molecule for nucleophilic attack at a carbonyl group on one prochiral face of the diketone substrate, effecting desymmetrizations of symmetrical substrates.

    Research areas

  • catalytic triad, crotonase, beta-diketone, dioxygenase, hydrolase, polyketide, POLYVINYL ALCOHOL)-DEGRADING ENZYME, RETRO-CLAISEN REACTION, CRYSTAL-STRUCTURE, CROTONASE SUPERFAMILY, PSEUDOMONAS SP, ANGSTROM RESOLUTION, ACINETOBACTER-JOHNSONII, SUBSTRATE-SPECIFICITY, DEGRADING ENZYME, CLEAVING ENZYME

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