Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme

Aelig Robin; Gareth A. Roberts; Johannes Kisch; Federico Sabbadin; Gideon Grogan; Neil Bruce; Nicholas J. Turner; Sabine L. Flitsch

doi:10.1039/b901716j

Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme

Aelig Robin, Gareth A. Roberts, Johannes Kisch, Federico Sabbadin, Gideon Grogan, Neil Bruce, Nicholas J. Turner, Sabine L. Flitsch

Research output: Contribution to journal › Article › peer-review

Abstract

A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor.

Original language	English
Pages (from-to)	2478-2480
Number of pages	3
Journal	Chemical Communications
Issue number	18
DOIs	https://doi.org/10.1039/b901716j
Publication status	Published - 2009

Keywords

ESCHERICHIA-COLI
RHODOCOCCUS SP
CYTOCHROME P450RHF
SYSTEM
FLAVOCYTOCHROME
EXPRESSION
ROLES

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10.1039/b901716j

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title = "Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme",

abstract = "A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor.",

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author = "Aelig Robin and Roberts, {Gareth A.} and Johannes Kisch and Federico Sabbadin and Gideon Grogan and Neil Bruce and Turner, {Nicholas J.} and Flitsch, {Sabine L.}",

year = "2009",

doi = "10.1039/b901716j",

language = "English",

pages = "2478--2480",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "The Royal Society of Chemistry",

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T1 - Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme

AU - Robin, Aelig

AU - Roberts, Gareth A.

AU - Kisch, Johannes

AU - Sabbadin, Federico

AU - Grogan, Gideon

AU - Bruce, Neil

AU - Turner, Nicholas J.

AU - Flitsch, Sabine L.

PY - 2009

Y1 - 2009

N2 - A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor.

AB - A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor.

KW - ESCHERICHIA-COLI

KW - RHODOCOCCUS SP

KW - CYTOCHROME P450RHF

KW - SYSTEM

KW - FLAVOCYTOCHROME

KW - EXPRESSION

KW - ROLES

UR - http://www.scopus.com/inward/record.url?scp=65349121795&partnerID=8YFLogxK

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DO - 10.1039/b901716j

M3 - Article

SN - 1359-7345

SP - 2478

EP - 2480

JO - Chemical Communications

JF - Chemical Communications

IS - 18

ER -

Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme

Abstract

Keywords

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