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Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme

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JournalChemical Communications
DatePublished - 2009
Issue number18
Number of pages3
Pages (from-to)2478-2480
Original languageEnglish

Abstract

A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor.

    Research areas

  • ESCHERICHIA-COLI, RHODOCOCCUS SP, CYTOCHROME P450RHF, SYSTEM, FLAVOCYTOCHROME, EXPRESSION, ROLES

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