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Enhanced Protein Extraction From Oilseed Cakes Using Glycerol-Choline Chloride Deep Eutectic Solvents: A Biorefinery Approach

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  • Avtar Singh Matharu
  • Eduardo MacEdo De Melo
  • Aleksandra Grudniewska
  • Radosław Gniłka
  • Angel Chan
  • Filip Boratyński


Publication details

JournalACS Sustainable Chemistry & Engineering
DateAccepted/In press - 25 Sep 2018
DateE-pub ahead of print (current) - 25 Sep 2018
Number of pages10
Early online date25/09/18
Original languageEnglish


The enhanced extraction of proteins from rapeseed cake (RC) and evening primrose cake (EC) using a glycerol-choline chloride deep eutectic solvent (Glyceline) is reported. Protein-rich precipitates were obtained by adding water (antisolvent) to the DES extract derived at different processing temperatures. The presence of proteins in precipitates has been confirmed by several techniques, such as: NMR, ATR-IR, TGA, CHN and SDS-PAGE. Yield of precipitates improved with increasing temperature of treatment, reaching a maximum of 20% and 35% at 140 °C from RC and EC, respectively. In general, the protein content of the extracts was ca. 40–50%, which is up to 20% more than the starting materials. SDS-PAGE confirmed that glyceline selectively extracted cruciferin proteins (ca. 16–33 kDa) from RC, while proteins with variable molecular weight (10–40 kDa) were identified in EC extracts. As potential application cruciferin-rich RCP60 and RCP100 could be incorporated into final food formulations as a source of protein due to its light colour

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© 2018 American Chemical Society. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.

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