Enzymatic microreactors in chemical analysis and kinetic studies

Research output: Contribution to journalLiterature review

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JournalBiotechnology advances
DatePublished - 2006
Issue number1
Volume24
Number of pages16
Pages (from-to)42-57
Original languageEnglish

Abstract

The fields of application of microreactors are becoming wider every year. A considerable number of papers have been published recently reporting successful application of enzymatic microreactors in chemistry and biochemistry. Most are devices with enzymes immobilized on beads or walls of microfluidic channels, whilst some use dissolved enzymes to run a reaction in the microfluidic system. Apart from model systems, mostly with glucose oxidase, horseradish peroxidase and alkaline phosphatase, the principal fields of application of microreactors are tryptic digestion of proteins and polymerase chain reaction in automated analyses of proteomic and genetic material, respectively. Enzymatic microreactors also facilitate characterization of enzyme activity as a function of substrate concentration, and enable fast screening of new biocatalysts and their substrates. They may constitute key parts of lab-on-a-chip and mu TAS, assisting the analysis of biomolecules. This review provides systematic coverage of examples of reports on enzymatic microreactors published recently, as well as relevant older papers. (C) 2005 Elsevier Inc. All rights reserved.

    Research areas

  • characterization of proteins and DNA, enzyme immobilization, lab-on-a-chip, microfluidic systems, microreactors, CAPILLARY-ZONE-ELECTROPHORESIS, SCANNING ELECTROCHEMICAL MICROSCOPY, ONLINE PROTEIN DIGESTION, PEPTIDE-MAPPING ANALYSIS, FLOW-INJECTION ANALYSIS, TOTAL ANALYSIS SYSTEMS, IMMOBILIZED ENZYME, MICROFLUIDIC SYSTEMS, MASS-SPECTROMETRY, POROUS SILICON

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