Epidermal growth factor upregulates motility of Mat-LyLu rat prostate cancer cells partially via voltage-gated Na+ channel activity

Yanning Ding, William J. Brackenbury, Pinar U. Onganer, Ximena Montano, Louise M. Porter, Lucy F. Bates, Mustafa B. A. Djamgoz

Research output: Contribution to journalArticlepeer-review

Abstract

The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage-gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation with EGF for 24 h more than doubled VGSC current density. Similar treatment with EGF significantly and dose-dependently enhanced the cells' migration through Transwell filters. Both the patch clamp recordings and the migration assay suggested that endogenous EGF played a similar role. Importantly, co-application of EGF and tetrodotoxin, a highly selective VGSC blocker, abolished 65% of the potentiating effect of EGF. It is suggested that a significant portion of the EGF-induced enhancement of migration occurred via VGSC activity.

Original languageEnglish
Pages (from-to)77-81
Number of pages5
JournalJournal of cellular physiology
Volume215
Issue number1
Early online date24 Oct 2007
DOIs
Publication statusPublished - Apr 2008

Bibliographical note

Copyright © 2007 Wiley-Liss, Inc. This is an author produced version of a paper published in Journal of Cellular Physiology. Uploaded in accordance with the publisher's self-archiving policy.

Keywords

  • FACTOR RECEPTOR
  • BREAST-CANCER
  • IN-VITRO
  • METASTATIC CASCADE
  • SODIUM-CHANNELS
  • LUNG-CANCER
  • FUNCTIONAL EXPRESSION
  • MEMBRANE-ACTIVITY
  • ELECTRIC-FIELD
  • LINE

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