Epidermal Notch1 recruits RORγ+ group 3 innate lymphoid cells to orchestrate normal skin repair

Zhi Li, Tom Hodgkinson, Elizabeth Jane Gothard, Soulmaz Boroumand, Ian Cummins, Priyanka Narang, Amy Kathleen Sawtell, Jenny Coles, German Leonov, Andrea Reboldi, Christopher Buckley, Tom Cupedo, Christian Siebel, Ardeshir Bayat, Mark Christopher Coles, Carrie Ambler

Research output: Contribution to journalArticlepeer-review


Notch has a well-defined role in controlling cell fate decisions in the embryo and the adult epidermis and immune systems, yet emerging evidence suggests Notch also directs non-cell-autonomous signalling in adult tissues. Here, we show that Notch1 works as a damage response signal. Epidermal Notch induces recruitment of immune cell subsets including RORγ + ILC3s into wounded dermis; RORγ + ILC3s are potent sources of IL17F in wounds and control immunological and epidermal cell responses. Mice deficient for RORγ + ILC3s heal wounds poorly resulting from delayed epidermal proliferation and macrophage recruitment in a CCL3-dependent process. Notch1 upregulates TNFα and the ILC3 recruitment chemokines CCL20 and CXCL13. TNFα, as a Notch1 effector, directs ILC3 localization and rates of wound healing. Altogether these findings suggest that Notch is a key stress/injury signal in skin epithelium driving innate immune cell recruitment and normal skin tissue repair.

Original languageEnglish
Article number11394
Number of pages14
JournalNature Communications
Publication statusPublished - 21 Apr 2016


  • Animals
  • Cell Movement/immunology
  • Chemokine CCL20/genetics
  • Chemokine CXCL13/genetics
  • Epidermis/immunology
  • Female
  • Gene Expression Regulation
  • Immunity, Innate
  • Interleukin-17/genetics
  • Lymphocyte Subsets/metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Knockout
  • Nuclear Receptor Subfamily 1, Group F, Member 3/deficiency
  • Receptor, Notch1/genetics
  • Signal Transduction/immunology
  • Tumor Necrosis Factor-alpha/genetics
  • Wound Healing/genetics
  • Wounds, Penetrating/genetics

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