Evolutionary Relationships among Actinophages and a Putative Adaptation for Growth in Streptomyces spp

TY - JOUR

T1 - Evolutionary Relationships among Actinophages and a Putative Adaptation for Growth in Streptomyces spp

AU - Smith, Margaret C M

AU - Hendrix, Roger W

AU - Dedrick, Rebekah

AU - Mitchell, Kaitlin

AU - Ko, Ching-Chung

AU - Russell, Daniel

AU - Bell, Emma

AU - Gregory, Matthew

AU - Bibb, Maureen J

AU - Pethick, Florence

AU - Jacobs-Sera, Deborah

AU - Herron, Paul

AU - Buttner, Mark J

AU - Hatfull, Graham F

PY - 2013/8/30

Y1 - 2013/8/30

N2 - The genome sequences of eight Streptomyces phages are presented, four of which have been isolated for this study. Phages R4, TG1, ϕHau3 and SV1 were isolated previously and have been exploited as tools for understanding and genetically manipulating Streptomyces spp. We also extracted five apparently intact prophages from recent Streptomyces spp genome projects and, together with six phage genomes in the database, we analysed all nineteen Streptomyces phage genomes with a view to understanding their relationships to each other and to other actinophages, in particular the mycobacteriophages. Sixteen of the Streptomyces phages group into four clusters of related genomes. Although the R4-like phages do not share nucleotide sequence similarity with other phages, they clearly have common ancestry with Cluster A mycobacteriophages, sharing many protein homologues, common gene syntenies, and similar repressor-stoperator regulatory systems.. The R4-like phage, ϕHau3 and the prophage StrepC.1 (from Streptomyces sp. C) appear to have hijacked a unique adaptation of the streptomycetes, i.e., use of the rare UUA codon, to control translation of the essential phage protein, the terminase. The Streptomyces venezuelae generalised transducing phage, SV1, was used to predict the presence of other generalised transducing phages for different Streptomyces species.

AB - The genome sequences of eight Streptomyces phages are presented, four of which have been isolated for this study. Phages R4, TG1, ϕHau3 and SV1 were isolated previously and have been exploited as tools for understanding and genetically manipulating Streptomyces spp. We also extracted five apparently intact prophages from recent Streptomyces spp genome projects and, together with six phage genomes in the database, we analysed all nineteen Streptomyces phage genomes with a view to understanding their relationships to each other and to other actinophages, in particular the mycobacteriophages. Sixteen of the Streptomyces phages group into four clusters of related genomes. Although the R4-like phages do not share nucleotide sequence similarity with other phages, they clearly have common ancestry with Cluster A mycobacteriophages, sharing many protein homologues, common gene syntenies, and similar repressor-stoperator regulatory systems.. The R4-like phage, ϕHau3 and the prophage StrepC.1 (from Streptomyces sp. C) appear to have hijacked a unique adaptation of the streptomycetes, i.e., use of the rare UUA codon, to control translation of the essential phage protein, the terminase. The Streptomyces venezuelae generalised transducing phage, SV1, was used to predict the presence of other generalised transducing phages for different Streptomyces species.

U2 - 10.1128/JB.00618-13

DO - 10.1128/JB.00618-13

M3 - Article

C2 - 23995638

SN - 0021-9193

JO - Journal of Bacteriology

JF - Journal of Bacteriology

ER -