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Exploring the biochemical properties and remediation applications of the unusual explosive-degrading P450 system XplA/B

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Exploring the biochemical properties and remediation applications of the unusual explosive-degrading P450 system XplA/B. / Jackson, R.G.; Rylott, E.L.; Fournier, D.; Hawari, J.; Bruce, N.C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 43, 10.2007, p. 16822-16827.

Research output: Contribution to journalArticle

Harvard

Jackson, RG, Rylott, EL, Fournier, D, Hawari, J & Bruce, NC 2007, 'Exploring the biochemical properties and remediation applications of the unusual explosive-degrading P450 system XplA/B', Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 43, pp. 16822-16827. https://doi.org/10.1073/pnas.0705110104

APA

Jackson, R. G., Rylott, E. L., Fournier, D., Hawari, J., & Bruce, N. C. (2007). Exploring the biochemical properties and remediation applications of the unusual explosive-degrading P450 system XplA/B. Proceedings of the National Academy of Sciences of the United States of America, 104(43), 16822-16827. https://doi.org/10.1073/pnas.0705110104

Vancouver

Jackson RG, Rylott EL, Fournier D, Hawari J, Bruce NC. Exploring the biochemical properties and remediation applications of the unusual explosive-degrading P450 system XplA/B. Proceedings of the National Academy of Sciences of the United States of America. 2007 Oct;104(43):16822-16827. https://doi.org/10.1073/pnas.0705110104

Author

Jackson, R.G. ; Rylott, E.L. ; Fournier, D. ; Hawari, J. ; Bruce, N.C. / Exploring the biochemical properties and remediation applications of the unusual explosive-degrading P450 system XplA/B. In: Proceedings of the National Academy of Sciences of the United States of America. 2007 ; Vol. 104, No. 43. pp. 16822-16827.

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@article{d5d7471f03c840a98a138925118d4301,
title = "Exploring the biochemical properties and remediation applications of the unusual explosive-degrading P450 system XplA/B",
abstract = "Widespread contamination of land and groundwater has resulted from the use, manufacture, and storage of the military explosive hexa-hydro-1,3,5-trinitro-1,3,5-triazine (RDX). This contamination has led to a requirement for a sustainable, low-cost method to remediate this problem. Here, we present the characterization of an unusual microbial P450 system able to degrade RDX, consisting of flavodoxin reductase XplB and fused flavodoxin-cytochrome P450 XplA. The affinity of XplA for the xenobiotic compound RDX is high (K d = 58 µM) and comparable with the K m of other P450s toward their natural substrates (ranging from 1 to 500 µM). The maximum turnover (k cat) is 4.44 per s, only 10-fold less than the fastest self-sufficient P450 reported, BM3. Interestingly, the presence of oxygen determines the final products of RDX degradation, demonstrating that the degradation chemistry is flexible, but both pathways result in ring cleavage and release of nitrite. Carbon monoxide inhibition is weak and yet the nitroaromatic explosive 2,4,6-trinitrotoluene (TNT) is a potent inhibitor. To test the efficacy of this system for the remediation of groundwater, transgenic Arabidopsis plants expressing both xplA and xplB were generated. They are able to remove saturating levels of RDX from liquid culture and soil leachate at rates significantly faster than those of untransformed plants and xplA-only transgenic lines, demonstrating the applicability of this system for the phytoremediation of RDX-contaminated sites.",
author = "R.G. Jackson and E.L. Rylott and D. Fournier and J. Hawari and N.C. Bruce",
year = "2007",
month = "10",
doi = "10.1073/pnas.0705110104",
language = "English",
volume = "104",
pages = "16822--16827",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "43",

}

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TY - JOUR

T1 - Exploring the biochemical properties and remediation applications of the unusual explosive-degrading P450 system XplA/B

AU - Jackson, R.G.

AU - Rylott, E.L.

AU - Fournier, D.

AU - Hawari, J.

AU - Bruce, N.C.

PY - 2007/10

Y1 - 2007/10

N2 - Widespread contamination of land and groundwater has resulted from the use, manufacture, and storage of the military explosive hexa-hydro-1,3,5-trinitro-1,3,5-triazine (RDX). This contamination has led to a requirement for a sustainable, low-cost method to remediate this problem. Here, we present the characterization of an unusual microbial P450 system able to degrade RDX, consisting of flavodoxin reductase XplB and fused flavodoxin-cytochrome P450 XplA. The affinity of XplA for the xenobiotic compound RDX is high (K d = 58 µM) and comparable with the K m of other P450s toward their natural substrates (ranging from 1 to 500 µM). The maximum turnover (k cat) is 4.44 per s, only 10-fold less than the fastest self-sufficient P450 reported, BM3. Interestingly, the presence of oxygen determines the final products of RDX degradation, demonstrating that the degradation chemistry is flexible, but both pathways result in ring cleavage and release of nitrite. Carbon monoxide inhibition is weak and yet the nitroaromatic explosive 2,4,6-trinitrotoluene (TNT) is a potent inhibitor. To test the efficacy of this system for the remediation of groundwater, transgenic Arabidopsis plants expressing both xplA and xplB were generated. They are able to remove saturating levels of RDX from liquid culture and soil leachate at rates significantly faster than those of untransformed plants and xplA-only transgenic lines, demonstrating the applicability of this system for the phytoremediation of RDX-contaminated sites.

AB - Widespread contamination of land and groundwater has resulted from the use, manufacture, and storage of the military explosive hexa-hydro-1,3,5-trinitro-1,3,5-triazine (RDX). This contamination has led to a requirement for a sustainable, low-cost method to remediate this problem. Here, we present the characterization of an unusual microbial P450 system able to degrade RDX, consisting of flavodoxin reductase XplB and fused flavodoxin-cytochrome P450 XplA. The affinity of XplA for the xenobiotic compound RDX is high (K d = 58 µM) and comparable with the K m of other P450s toward their natural substrates (ranging from 1 to 500 µM). The maximum turnover (k cat) is 4.44 per s, only 10-fold less than the fastest self-sufficient P450 reported, BM3. Interestingly, the presence of oxygen determines the final products of RDX degradation, demonstrating that the degradation chemistry is flexible, but both pathways result in ring cleavage and release of nitrite. Carbon monoxide inhibition is weak and yet the nitroaromatic explosive 2,4,6-trinitrotoluene (TNT) is a potent inhibitor. To test the efficacy of this system for the remediation of groundwater, transgenic Arabidopsis plants expressing both xplA and xplB were generated. They are able to remove saturating levels of RDX from liquid culture and soil leachate at rates significantly faster than those of untransformed plants and xplA-only transgenic lines, demonstrating the applicability of this system for the phytoremediation of RDX-contaminated sites.

U2 - 10.1073/pnas.0705110104

DO - 10.1073/pnas.0705110104

M3 - Article

VL - 104

SP - 16822

EP - 16827

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 43

ER -