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Expression and cytokine regulation of immune recognition elements by normal human biliary epithelial and established liver cell lines in vitro

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JournalJournal of Hepatology
DatePublished - 1 Oct 1998
Issue number4
Volume29
Number of pages9
Pages (from-to)550-558
Original languageEnglish

Abstract

Background/Aims: Biliary epithelial cells are targets of immune-mediated attack in conditions such as primary biliary cirrhosis and allograft rejection. This has been attributed to the ability of biliary epithelial cells to express ligands for T cell receptors. We aimed to investigate the expression of immune recognition elements and the effects of pro-inflammatory and antiinflammatory cytokines on cell surface phenotypes of normal human biliary epithelial cells and established human liver-derived (PLC/PRF/5, HepG2, Hep3B and CC-SW) lines. Methods: Cells were cultured in the presence or absence of cytokines for 72 h, and expression of cell surface molecules was assessed by flow cytometry and immunofluorescence. Results: All cell lines expressed MHC class I, ICAM1 (CD54), LFA-3 (CD58) and EGF receptor, and all but Hep3B expressed Fas/Apo-1 (CD95). Unlike hepatocyte-derived cell lines, biliary epithelial cells and CC-SW expressed CD40 and CD44. As expected, IFNγ and TNFα upregulated expression of ICAM-1, MHC class I and MHC class II, particularly in biliary epithelial cells. TGFβ downregulated these molecules and downregulated CD95 on biliary epithelial cells, but upregulated LFA-3. The Th2 cytokines had little effect, although IL-4 upregulated CD95 expression on biliary epithelial cells. IFNγ upregulated CD40 expression on biliary epithelial cells, CC-SW and HepG2. Conclusions: These findings imply that biliary epithelial cells may be capable of interacting with activated T lymphocytes via CD40 and LFA-3, which are thought to be important T cell accessory ligands for T cell activation in a B7-independent manner. Sensitivity to pro-inflammatory cytokines and expression of CD95 may explain why biliary epithelial cells are primary targets for autoimmune attack.

    Research areas

  • Biliary, CD40, CD44, CD95, Cytokines, Epithelia, Immunoregulation

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