Expression and localization of endothelin-converting enzyme-1 in human prostate cancer

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Expression and localization of endothelin-converting enzyme-1 in human prostate cancer. / Dawson, L A; Maitland, N J; Berry, P; Turner, A J; Usmani, B A.

In: Experimental biology and medicine, Vol. 231, No. 6, 06.2006, p. 1106-1110.

Research output: Contribution to journalArticle

Harvard

Dawson, LA, Maitland, NJ, Berry, P, Turner, AJ & Usmani, BA 2006, 'Expression and localization of endothelin-converting enzyme-1 in human prostate cancer', Experimental biology and medicine, vol. 231, no. 6, pp. 1106-1110.

APA

Dawson, L. A., Maitland, N. J., Berry, P., Turner, A. J., & Usmani, B. A. (2006). Expression and localization of endothelin-converting enzyme-1 in human prostate cancer. Experimental biology and medicine, 231(6), 1106-1110.

Vancouver

Dawson LA, Maitland NJ, Berry P, Turner AJ, Usmani BA. Expression and localization of endothelin-converting enzyme-1 in human prostate cancer. Experimental biology and medicine. 2006 Jun;231(6):1106-1110.

Author

Dawson, L A ; Maitland, N J ; Berry, P ; Turner, A J ; Usmani, B A. / Expression and localization of endothelin-converting enzyme-1 in human prostate cancer. In: Experimental biology and medicine. 2006 ; Vol. 231, No. 6. pp. 1106-1110.

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@article{af00a2d44d7f43c9af63bbcaa7193d8e,
title = "Expression and localization of endothelin-converting enzyme-1 in human prostate cancer",
abstract = "Endothelin (ET)-1 can influence cancer invasion and metastasis by exerting an autocrine (epithelial) or paracrine (stromal) influence on growth. ET-1 is generated from big ET-1 by endothelin-converting enzyme (ECE)-1, which has four recognized isoforms, ECE-1a, ECE-1b, ECE-1c, and ECE-1d, differing only in their amino-terminal regions. This study investigated the expression and localization of the ECE-1 isoforms in prostate cancer (PC). The epithelial cell lines used were androgen-sensitive LNCaP, androgen-independent PC-3 and Du145, and nonmalignant transformed PNT1-a, PNT2-C2, and P4E6 prostate cells. Primary cells derived from malignant and benign tissue from radical prostatectomies were also exploited. Previously, we reported increased ECE-1 expression in androgen-independent PC cell lines, as compared with androgen-sensitive cells. Our present data show that transcripts for all ECE-1 isoforms were present in all epithelial cell lines analyzed. However, only the ECE-1c protein was detectable in PC-3, Du145, PNT2-C2, and PNT1-a cells. ECE-1c localized to both the cell surface and intracellular compartments in individual cell lines. In primary stromal cells, all individual ECE-1 isoforms were expressed at the mRNA level, with the exception of ECE-1 a. ECE-1b and ECE1c protein levels were higher in malignant stromal cells, as compared with benign cells. In stroma, ECE-1c protein was localized to the cell surface, with filamentous immunoreactivity throughout the cell, whereas ECE-1b immunoreactivity was punctate throughout the cytoplasm. The upregulation of the ECE-1c isoform in PC cell lines is being investigated further.",
keywords = "endothelin-converting enzyme (ECE), isoforms, prostate cancer, stromal-epithelial interactions, metallopeptidases, endothelin, STROMAL INTERACTIONS, ISOFORMS, CELLS, RAT, METALLOPROTEASE, MEMBRANE, ECE-1",
author = "Dawson, {L A} and Maitland, {N J} and P Berry and Turner, {A J} and Usmani, {B A}",
year = "2006",
month = jun,
language = "English",
volume = "231",
pages = "1106--1110",
journal = "Experimental biology and medicine",
issn = "1535-3702",
publisher = "SAGE Publications Ltd",
number = "6",

}

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TY - JOUR

T1 - Expression and localization of endothelin-converting enzyme-1 in human prostate cancer

AU - Dawson, L A

AU - Maitland, N J

AU - Berry, P

AU - Turner, A J

AU - Usmani, B A

PY - 2006/6

Y1 - 2006/6

N2 - Endothelin (ET)-1 can influence cancer invasion and metastasis by exerting an autocrine (epithelial) or paracrine (stromal) influence on growth. ET-1 is generated from big ET-1 by endothelin-converting enzyme (ECE)-1, which has four recognized isoforms, ECE-1a, ECE-1b, ECE-1c, and ECE-1d, differing only in their amino-terminal regions. This study investigated the expression and localization of the ECE-1 isoforms in prostate cancer (PC). The epithelial cell lines used were androgen-sensitive LNCaP, androgen-independent PC-3 and Du145, and nonmalignant transformed PNT1-a, PNT2-C2, and P4E6 prostate cells. Primary cells derived from malignant and benign tissue from radical prostatectomies were also exploited. Previously, we reported increased ECE-1 expression in androgen-independent PC cell lines, as compared with androgen-sensitive cells. Our present data show that transcripts for all ECE-1 isoforms were present in all epithelial cell lines analyzed. However, only the ECE-1c protein was detectable in PC-3, Du145, PNT2-C2, and PNT1-a cells. ECE-1c localized to both the cell surface and intracellular compartments in individual cell lines. In primary stromal cells, all individual ECE-1 isoforms were expressed at the mRNA level, with the exception of ECE-1 a. ECE-1b and ECE1c protein levels were higher in malignant stromal cells, as compared with benign cells. In stroma, ECE-1c protein was localized to the cell surface, with filamentous immunoreactivity throughout the cell, whereas ECE-1b immunoreactivity was punctate throughout the cytoplasm. The upregulation of the ECE-1c isoform in PC cell lines is being investigated further.

AB - Endothelin (ET)-1 can influence cancer invasion and metastasis by exerting an autocrine (epithelial) or paracrine (stromal) influence on growth. ET-1 is generated from big ET-1 by endothelin-converting enzyme (ECE)-1, which has four recognized isoforms, ECE-1a, ECE-1b, ECE-1c, and ECE-1d, differing only in their amino-terminal regions. This study investigated the expression and localization of the ECE-1 isoforms in prostate cancer (PC). The epithelial cell lines used were androgen-sensitive LNCaP, androgen-independent PC-3 and Du145, and nonmalignant transformed PNT1-a, PNT2-C2, and P4E6 prostate cells. Primary cells derived from malignant and benign tissue from radical prostatectomies were also exploited. Previously, we reported increased ECE-1 expression in androgen-independent PC cell lines, as compared with androgen-sensitive cells. Our present data show that transcripts for all ECE-1 isoforms were present in all epithelial cell lines analyzed. However, only the ECE-1c protein was detectable in PC-3, Du145, PNT2-C2, and PNT1-a cells. ECE-1c localized to both the cell surface and intracellular compartments in individual cell lines. In primary stromal cells, all individual ECE-1 isoforms were expressed at the mRNA level, with the exception of ECE-1 a. ECE-1b and ECE1c protein levels were higher in malignant stromal cells, as compared with benign cells. In stroma, ECE-1c protein was localized to the cell surface, with filamentous immunoreactivity throughout the cell, whereas ECE-1b immunoreactivity was punctate throughout the cytoplasm. The upregulation of the ECE-1c isoform in PC cell lines is being investigated further.

KW - endothelin-converting enzyme (ECE)

KW - isoforms

KW - prostate cancer

KW - stromal-epithelial interactions

KW - metallopeptidases

KW - endothelin

KW - STROMAL INTERACTIONS

KW - ISOFORMS

KW - CELLS

KW - RAT

KW - METALLOPROTEASE

KW - MEMBRANE

KW - ECE-1

M3 - Article

VL - 231

SP - 1106

EP - 1110

JO - Experimental biology and medicine

JF - Experimental biology and medicine

SN - 1535-3702

IS - 6

ER -