Expression of an antigen associated with basal bodies of human ciliated epithelial cells

M T Comer, M Shires, N P Goode, H J Leese, L K Trejdosiewicz, J Southgate

Research output: Contribution to journalArticlepeer-review

Abstract

The process and regulation of ciliogenesis in human epithelia is little understood and many components of the cilium and associated structures have not been characterised. We have identified a monoclonal antibody, LhS28, which recognises a 44,000-45,000 M-r protein specifically associated with human ciliated epithelial cells. Immunoperoxidase labelling of formalin-fixed paraffin wax-embedded human tissues showed that LhS28 was expressed in the sub-apical zone of ciliated epithelial cells of the Fallopian tube and upper respiratory tract, but not ciliated ependyma, non-ciliated epithelia or testis containing developing spermatozoa. Immunoelectron microscopy demonstrated that the antigen recognised by LhS28 was associated with the basal body structure of the cilium and specifically with the 9+0 microtubule arrays. LhS28 should be a useful tool in the identification of ciliated cells in pathological specimens and for investigating mechanisms of ciliogenesis.

Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalHistochemical journal
Volume31
Issue number1
Publication statusPublished - Jan 1999

Keywords

  • IMMUNOELECTRON MICROSCOPY
  • MONOCLONAL-ANTIBODIES
  • UTERINE CERVIX
  • CILIOGENESIS
  • METAPLASIA
  • INDUCTION
  • OVIDUCT
  • TISSUE

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