Expression of an antigen associated with basal bodies of human ciliated epithelial cells

M T Comer; M Shires; N P Goode; H J Leese; L K Trejdosiewicz; J Southgate

Expression of an antigen associated with basal bodies of human ciliated epithelial cells

M T Comer, M Shires, N P Goode, H J Leese, L K Trejdosiewicz, J Southgate

Jack Birch Unit

Research output: Contribution to journal › Article › peer-review

Abstract

The process and regulation of ciliogenesis in human epithelia is little understood and many components of the cilium and associated structures have not been characterised. We have identified a monoclonal antibody, LhS28, which recognises a 44,000-45,000 M-r protein specifically associated with human ciliated epithelial cells. Immunoperoxidase labelling of formalin-fixed paraffin wax-embedded human tissues showed that LhS28 was expressed in the sub-apical zone of ciliated epithelial cells of the Fallopian tube and upper respiratory tract, but not ciliated ependyma, non-ciliated epithelia or testis containing developing spermatozoa. Immunoelectron microscopy demonstrated that the antigen recognised by LhS28 was associated with the basal body structure of the cilium and specifically with the 9+0 microtubule arrays. LhS28 should be a useful tool in the identification of ciliated cells in pathological specimens and for investigating mechanisms of ciliogenesis.

Original language	English
Pages (from-to)	39-43
Number of pages	5
Journal	Histochemical journal
Volume	31
Issue number	1
Publication status	Published - Jan 1999

Keywords

IMMUNOELECTRON MICROSCOPY
MONOCLONAL-ANTIBODIES
UTERINE CERVIX
CILIOGENESIS
METAPLASIA
INDUCTION
OVIDUCT
TISSUE

Cite this

@article{4583d3f98de2496f92b726d3c1eb3da4,

title = "Expression of an antigen associated with basal bodies of human ciliated epithelial cells",

abstract = "The process and regulation of ciliogenesis in human epithelia is little understood and many components of the cilium and associated structures have not been characterised. We have identified a monoclonal antibody, LhS28, which recognises a 44,000-45,000 M-r protein specifically associated with human ciliated epithelial cells. Immunoperoxidase labelling of formalin-fixed paraffin wax-embedded human tissues showed that LhS28 was expressed in the sub-apical zone of ciliated epithelial cells of the Fallopian tube and upper respiratory tract, but not ciliated ependyma, non-ciliated epithelia or testis containing developing spermatozoa. Immunoelectron microscopy demonstrated that the antigen recognised by LhS28 was associated with the basal body structure of the cilium and specifically with the 9+0 microtubule arrays. LhS28 should be a useful tool in the identification of ciliated cells in pathological specimens and for investigating mechanisms of ciliogenesis.",

keywords = "IMMUNOELECTRON MICROSCOPY, MONOCLONAL-ANTIBODIES, UTERINE CERVIX, CILIOGENESIS, METAPLASIA, INDUCTION, OVIDUCT, TISSUE",

author = "Comer, {M T} and M Shires and Goode, {N P} and Leese, {H J} and Trejdosiewicz, {L K} and J Southgate",

year = "1999",

month = jan,

language = "English",

volume = "31",

pages = "39--43",

journal = "Histochemical journal",

issn = "0018-2214",

publisher = "Kluwer",

number = "1",

}

TY - JOUR

T1 - Expression of an antigen associated with basal bodies of human ciliated epithelial cells

AU - Comer, M T

AU - Shires, M

AU - Goode, N P

AU - Leese, H J

AU - Trejdosiewicz, L K

AU - Southgate, J

PY - 1999/1

Y1 - 1999/1

N2 - The process and regulation of ciliogenesis in human epithelia is little understood and many components of the cilium and associated structures have not been characterised. We have identified a monoclonal antibody, LhS28, which recognises a 44,000-45,000 M-r protein specifically associated with human ciliated epithelial cells. Immunoperoxidase labelling of formalin-fixed paraffin wax-embedded human tissues showed that LhS28 was expressed in the sub-apical zone of ciliated epithelial cells of the Fallopian tube and upper respiratory tract, but not ciliated ependyma, non-ciliated epithelia or testis containing developing spermatozoa. Immunoelectron microscopy demonstrated that the antigen recognised by LhS28 was associated with the basal body structure of the cilium and specifically with the 9+0 microtubule arrays. LhS28 should be a useful tool in the identification of ciliated cells in pathological specimens and for investigating mechanisms of ciliogenesis.

AB - The process and regulation of ciliogenesis in human epithelia is little understood and many components of the cilium and associated structures have not been characterised. We have identified a monoclonal antibody, LhS28, which recognises a 44,000-45,000 M-r protein specifically associated with human ciliated epithelial cells. Immunoperoxidase labelling of formalin-fixed paraffin wax-embedded human tissues showed that LhS28 was expressed in the sub-apical zone of ciliated epithelial cells of the Fallopian tube and upper respiratory tract, but not ciliated ependyma, non-ciliated epithelia or testis containing developing spermatozoa. Immunoelectron microscopy demonstrated that the antigen recognised by LhS28 was associated with the basal body structure of the cilium and specifically with the 9+0 microtubule arrays. LhS28 should be a useful tool in the identification of ciliated cells in pathological specimens and for investigating mechanisms of ciliogenesis.

KW - IMMUNOELECTRON MICROSCOPY

KW - MONOCLONAL-ANTIBODIES

KW - UTERINE CERVIX

KW - CILIOGENESIS

KW - METAPLASIA

KW - INDUCTION

KW - OVIDUCT

KW - TISSUE

M3 - Article

SN - 0018-2214

VL - 31

SP - 39

EP - 43

JO - Histochemical journal

JF - Histochemical journal

IS - 1

ER -